Cholesterol oxidase
From Proteopedia
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| - | + | <StructureSection load='1coy' size='350' side='right' caption='Cholesterol oxidase complex with steroid substrate (PDB entry [[1coy]])' scene='48/486379/Cv/1'> | |
| - | + | == Function == | |
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| + | '''Cholesterol oxidase''' (COX) catalyzes the conversion of cholesterol and molecular oxygen to Colest-4-en-3-one and hydrogen peroxide. COX uses FAD as a cofactor. '''COX I''' binds FAD non-covalently whereas '''COX II''' binds FAD covalently.<ref>PMID:20102741</ref> | ||
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| + | == Relevance == | ||
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| + | COX is used as a biosensor for the detection of cholesterol in blood serum and food. | ||
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| + | == Structural highlights == | ||
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| + | There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/7'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/8'>FAD binding site</scene> (PDB entry [[1coy]]).<ref>PMID:8218217</ref> Water molecules are shown as red spheres. <scene name='48/486379/Cv/9'>Whole active site</scene>. | ||
| + | </StructureSection> | ||
==3D structure of cholesterol oxidase== | ==3D structure of cholesterol oxidase== | ||
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*Cholesterol oxidase | *Cholesterol oxidase | ||
| - | **[[ | + | **[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br /> |
| - | **[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]] – StCOX – ''Streptomyces''<br /> | + | **[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]], [[5kwf]], [[4xxg]], [[4xwr]], [[4rek]] – StCOX – ''Streptomyces''<br /> |
**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br /> | **[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br /> | ||
| - | **[[3js8]] – COX – ''Chromobacterium'' | + | **[[3js8]] – COX – ''Chromobacterium''<br /> |
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*Cholesterol oxidase complex | *Cholesterol oxidase complex | ||
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**[[3b3r]] - StCOX (mutant) + glycerol | **[[3b3r]] - StCOX (mutant) + glycerol | ||
}} | }} | ||
| + | == References == | ||
| + | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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3D structure of cholesterol oxidase
Updated on 12-May-2019
References
- ↑ Sagermann M, Ohtaki A, Newton K, Doukyu N. Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp. DS-1. J Struct Biol. 2010 Apr;170(1):32-40. Epub 2010 Jan 25. PMID:20102741 doi:10.1016/j.jsb.2010.01.012
- ↑ Li J, Vrielink A, Brick P, Blow DM. Crystal structure of cholesterol oxidase complexed with a steroid substrate: implications for flavin adenine dinucleotide dependent alcohol oxidases. Biochemistry. 1993 Nov 2;32(43):11507-15. PMID:8218217
