4wz9
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4wz9 is ON HOLD Authors: Atkinson, S.C., Armistead, J.S., Mathias, D.K., Sandeu, M.M., Tao, D., Borhani-Dizaji, N., Morlais, I., Dinglasan, R.R., Bo...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==APN1 from Anopheles gambiae== | |
| + | <StructureSection load='4wz9' size='340' side='right'caption='[[4wz9]], [[Resolution|resolution]] 2.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wz9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae] and [https://en.wikipedia.org/wiki/Drosophila Drosophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WZ9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wz9 OCA], [https://pdbe.org/4wz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wz9 RCSB], [https://www.ebi.ac.uk/pdbsum/4wz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wz9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/AMPN_ANOGA AMPN_ANOGA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mosquito-based malaria transmission-blocking vaccines (mTBVs) target midgut-surface antigens of the Plasmodium parasite's obligate vector, the Anopheles mosquito. The alanyl aminopeptidase N (AnAPN1) is the leading mTBV immunogen; however, AnAPN1's role in Plasmodium infection of the mosquito and how anti-AnAPN1 antibodies functionally block parasite transmission have remained elusive. Here we present the 2.65-A crystal structure of AnAPN1 and the immunoreactivity and transmission-blocking profiles of three monoclonal antibodies (mAbs) to AnAPN1, including mAb 4H5B7, which effectively blocks transmission of natural strains of Plasmodium falciparum. Using the AnAPN1 structure, we map the conformation-dependent 4H5B7 neoepitope to a previously uncharacterized region on domain 1 and further demonstrate that nonhuman-primate neoepitope-specific IgG also blocks parasite transmission. We discuss the prospect of a new biological function of AnAPN1 as a receptor for Plasmodium in the mosquito midgut and the implications for redesigning the AnAPN1 mTBV. | ||
| - | + | The Anopheles-midgut APN1 structure reveals a new malaria transmission-blocking vaccine epitope.,Atkinson SC, Armistead JS, Mathias DK, Sandeu MM, Tao D, Borhani-Dizaji N, Tarimo BB, Morlais I, Dinglasan RR, Borg NA Nat Struct Mol Biol. 2015 Jun 15. doi: 10.1038/nsmb.3048. PMID:26075520<ref>PMID:26075520</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4wz9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Anopheles gambiae]] | ||
| + | [[Category: Drosophila]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Armistead JS]] | ||
| + | [[Category: Atkinson SC]] | ||
| + | [[Category: Borg NA]] | ||
| + | [[Category: Borhani-Dizaji N]] | ||
| + | [[Category: Dinglasan RR]] | ||
| + | [[Category: Mathias DK]] | ||
| + | [[Category: Morlais I]] | ||
| + | [[Category: Sandeu MM]] | ||
| + | [[Category: Tao D]] | ||
Current revision
APN1 from Anopheles gambiae
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