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1lwl

From Proteopedia

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[[Image:1lwl.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)==
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|PDB= 1lwl |SIZE=350|CAPTION= <scene name='initialview01'>1lwl</scene>, resolution 2.2&Aring;
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<StructureSection load='1lwl' size='340' side='right'caption='[[1lwl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=DSO:ADAMANTANE-1-CARBOXYLIC ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-OCTYL-AMIDE'>DSO</scene>
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<table><tr><td colspan='2'>[[1lwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LWL FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSO:ADAMANTANE-1-CARBOXYLIC+ACID-5-DIMETHYLAMINO-NAPHTHALENE-1-SULFONYLAMINO-OCTYL-AMIDE'>DSO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lwl OCA], [https://pdbe.org/1lwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lwl RCSB], [https://www.ebi.ac.uk/pdbsum/1lwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lwl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPXA_PSEPU CPXA_PSEPU] Involved in a camphor oxidation system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/1lwl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lwl ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)'''
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==See Also==
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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We have synthesized two luminescent probes (D-4-Ad and D-8-Ad) that target cytochrome P450cam. D-4-Ad luminescence is quenched by Forster energy transfer upon binding (Kd = 0.83 muM) but is restored when the probe is displaced from the active site by camphor. In contrast, D-8-Ad (Kd approximately 0.02 muM) is not displaced from the enzyme, even in the presence of a large excess of camphor. The 2.2 A resolution crystal structure of the D-8-Ad:P450cam complex reveals extensive hydrophobic contacts between the probe and the enzyme, which result from the conformational flexibility of the B', F, and G helices. Probes with properties similar to those of D-4-Ad potentially could be useful for screening P450 inhibitors.
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[[Category: Large Structures]]
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==About this Structure==
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1LWL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWL OCA].
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==Reference==
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Fluorescent probes for cytochrome p450 structural characterization and inhibitor screening., Dunn AR, Hays AM, Goodin DB, Stout CD, Chiu R, Winkler JR, Gray HB, J Am Chem Soc. 2002 Sep 4;124(35):10254-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12197708 12197708]
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[[Category: Camphor 5-monooxygenase]]
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[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: Single protein]]
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[[Category: Chiu R]]
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[[Category: Chiu, R.]]
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[[Category: Dunn AR]]
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[[Category: Dunn, A R.]]
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[[Category: Goodin DB]]
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[[Category: Goodin, D B.]]
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[[Category: Gray HB]]
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[[Category: Gray, H B.]]
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[[Category: Hays AM]]
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[[Category: Hays, A M.]]
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[[Category: Stout CD]]
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[[Category: Stout, C D.]]
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[[Category: Winkler JR]]
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[[Category: Winkler, J R.]]
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[[Category: DSO]]
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[[Category: HEM]]
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[[Category: adamantane]]
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[[Category: adamantane-1-carboxylic acid [4-(5-dimethylamino-naphthalene-1-sulfonylamino)-octyl]-amide]]
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[[Category: channel]]
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[[Category: dansyl]]
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[[Category: electron transfer]]
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[[Category: energy transfer]]
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[[Category: monooxygenase]]
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[[Category: substrate-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:36:04 2008''
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Current revision

Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)

PDB ID 1lwl

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