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Glycerol kinase

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{{STRUCTURE_1r59| PDB=1r59 | SIZE=400| SCENE= |right| CAPTION=Glycerol kinase dimer [[1r59]] }}
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<StructureSection load='' size='350' side='right' caption='Glycerol kinase complex with glycerol (PDB entry [[1xup]])' scene='48/487479/Cv/1'>
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== Function ==
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'''Glycerol kinase''' (GK) phosphorylates glycerol forming glycerol 3-phosphate (G3P) using Mg-ATP as phosphate source. GK is a key enzyme in glycerol uptake and metabolism. Mutations of GK gene cause GK deficiency syndrome. PK is a multi-subunit allosteric enzyme. Its activity can be inhibited by fructose 1,6-bisphosphate (FBP) and by the glucose-specific phosphocarrier IIA(Glc). PK cofactor is a Zn atom which binds to the dimer<ref>PMID:10759857</ref>.
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== Disease ==
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GK Deficiency is an X-linked disease which has 3 forms: infantile, juvenile and adult<ref>PMID:15684452</ref>
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'''Glycerol kinase''' (GK) phosphorylates glycerol forming glycerol 3-phosphate (G3P) using Mg-ATP as phosphate source. GK is a key enzyme in glycerol uptake and metabolism. Mutations of GK gene cause GK deficiency syndrome. PK is a multi-subunit allosteric enzyme. Its activity can be inhibited by fructose 1,6-bisphosphate (FBP) and by the glucose-specific phosphocarrier IIA(Glc). PK cofactor is a Zn atom which binds to the dimer.
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== Structural highlights ==
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<scene name='48/487479/Cv/3'>GK active site</scene> is located in a cleft between two domains<ref>PMID:14717590</ref>.
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</StructureSection>
==3D structures of glycerol kinase==
==3D structures of glycerol kinase==
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**[[2dpn]] - GK - ''Thermus thermophilus''<br />
**[[2dpn]] - GK - ''Thermus thermophilus''<br />
**[[2d4w]] - GK - ''Cellulomonas''<br />
**[[2d4w]] - GK - ''Cellulomonas''<br />
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**[[2zf5]] - GK - ''Thermococcus kodakarensis''<br />
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**[[2zf5]] - TkGK - ''Thermococcus kodakarensis''<br />
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**[[3gg4]] – GK – ''Yersinia pseudotuberculosis''
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**[[3gg4]] – GK – ''Yersinia pseudotuberculosis''<br />
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**[[3wxi]] – TbGK – ''Trypanosoma brucei gambiense''<br />
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**[[6zq5]], [[6zq8]] – CtGK – ''Chaetonium thermophilum'' <br />
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*Glycerol kinase binary complex with glycerol
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*Glycerol kinase binary complex
**[[1xup]] - EncGK + glycerol<br />
**[[1xup]] - EncGK + glycerol<br />
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**[[4e1j]] – GK + glycerol – ''Sinorhizobium meliloti''<br />
**[[4e1j]] – GK + glycerol – ''Sinorhizobium meliloti''<br />
**[[1bu6]], [[3ezw]] - EcGK (mutant) + glycerol<br />
**[[1bu6]], [[3ezw]] - EcGK (mutant) + glycerol<br />
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**[[3wxk]], [[6j9x]], [[6jae]], [[6jaf]] – TbGK + glycerol <br />
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**[[6zq4]], [[6zq6]], [[6zq7]] – CtGK + glycerol <br />
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**[[6k76]] – TkGK + AMPPNP<br />
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**[[6k78]], [[6k79]] – TkGK + glycerol <br />
*Glycerol kinase ternary complex
*Glycerol kinase ternary complex
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**[[3ge1]] - SaGK + ADP + glycerol<br />
**[[3ge1]] - SaGK + ADP + glycerol<br />
**[[3h3o]] - EncGK (mutant) + phosphate + glycerol<br />
**[[3h3o]] - EncGK (mutant) + phosphate + glycerol<br />
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**[[3h45]] - EncGK (mutant) + phosphate + ethylene glycol
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**[[3h45]] - EncGK (mutant) + phosphate + ethylene glycol<br />
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**[[3wxj]] – TbGK + glycerol + G3P<br />
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**[[3wxl]], [[6j9v]] – TbGK + glycerol + ADP<br />
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**[[6j9q]] – TbGK + glycerol + AMPPNP<br />
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**[[5azi]], [[5azj]] – TbGK + glycerol + nitrophenyl-phosphate<br />
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**[[5gn5]], [[5gn6]] – TbGK + glycerol + coumarin derivative<br />
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**[[6ude]] – GK + glycerol + ADP – ''Elizabethkingia anophelis''<br />
}}
}}
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Glycerol kinase complex with glycerol (PDB entry 1xup)

Drag the structure with the mouse to rotate

3D structures of glycerol kinase

Updated on 11-August-2021

References

  1. ↑ Kralova I, Rigden DJ, Opperdoes FR, Michels PA. Glycerol kinase of Trypanosoma brucei. Cloning, molecular characterization and mutagenesis. Eur J Biochem. 2000 Apr;267(8):2323-33. PMID:10759857
  2. ↑ Sehgal A, Stack J. Complex glycerol kinase deficiency: an X-linked disorder associated with adrenal hypoplasia congenita. Indian J Pediatr. 2005 Jan;72(1):67-9. PMID:15684452
  3. ↑ Yeh JI, Charrier V, Paulo J, Hou L, Darbon E, Claiborne A, Hol WG, Deutscher J. Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation. Biochemistry. 2004 Jan 20;43(2):362-73. PMID:14717590 doi:10.1021/bi034258o

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