1m55

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[[Image:1m55.gif|left|200px]]
 
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{{Structure
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==Catalytic domain of the Adeno Associated Virus type 5 Rep protein==
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|PDB= 1m55 |SIZE=350|CAPTION= <scene name='initialview01'>1m55</scene>, resolution 1.40&Aring;
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<StructureSection load='1m55' size='340' side='right'caption='[[1m55]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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<table><tr><td colspan='2'>[[1m55]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_5 Adeno-associated virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M55 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m55 OCA], [https://pdbe.org/1m55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m55 RCSB], [https://www.ebi.ac.uk/pdbsum/1m55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m55 ProSAT]</span></td></tr>
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</table>
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'''Catalytic domain of the Adeno Associated Virus type 5 Rep protein'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q9YJC1_9VIRU Q9YJC1_9VIRU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Adeno-associated virus (AAV), unique among animal viruses in its ability to integrate into a specific chromosomal location, is a promising vector for human gene therapy. AAV Replication (Rep) protein is essential for viral replication and integration, and its amino terminal domain possesses site-specific DNA binding and endonuclease activities required for replication initiation and integration. This domain displays a novel endonuclease fold and demonstrates an unexpected structural relationship to other viral origin binding proteins such as the papillomavirus E1 protein and the SV40 T antigen. The active site, located at the bottom of a positively charged cleft, is formed by the spatial convergence of a divalent metal ion and two conserved sequence motifs that define the rolling circle replication superfamily.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/1m55_consurf.spt"</scriptWhenChecked>
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1M55 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M55 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural unity among viral origin binding proteins: crystal structure of the nuclease domain of adeno-associated virus Rep., Hickman AB, Ronning DR, Kotin RM, Dyda F, Mol Cell. 2002 Aug;10(2):327-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12191478 12191478]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m55 ConSurf].
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[[Category: Adeno-associated virus - 5]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Dyda, F.]]
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</StructureSection>
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[[Category: Hickman, A B.]]
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[[Category: Adeno-associated virus 5]]
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[[Category: Kotin, R M.]]
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[[Category: Large Structures]]
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[[Category: Ronning, D R.]]
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[[Category: Dyda F]]
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[[Category: CL]]
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[[Category: Hickman AB]]
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[[Category: ZN]]
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[[Category: Kotin RM]]
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[[Category: adeno-associated virus]]
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[[Category: Ronning DR]]
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[[Category: endonuclease]]
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[[Category: rep]]
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[[Category: rolling circle replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:38:59 2008''
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Current revision

Catalytic domain of the Adeno Associated Virus type 5 Rep protein

PDB ID 1m55

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OCA

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