MECDP synthase

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{{STRUCTURE_1gx1| PDB=1gx1 | SIZE=400| SCENE= |right|CAPTION=Se-Met MECDP synthase trimer complex with CDP (stick model), sulfate, Mn+2 (purple) and Zn+2 (grey) ion, [[1gx1]] }}
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<StructureSection load='1gx1' size='450' side='right' scene='52/526157/Cv/1' caption='Se-Met MECDP synthase trimer complex with CDP, sulfate, Mn+2 (purple) and Zn+2 (grey) ion, [[1gx1]]'>
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'''MECDP synthase''' (MECPS) participates in mevalonate-independent steroid biosynthesis. It converts 2-phospho-4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol (2P-CDME) to 2-C-methyl-D-erythritol 2, 4-cyclodiphosphate (MECP) and CMP. MECPS contains metal binding sites which are required for catalysis. MECPS is a potential antimalarial drug target.
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== Function ==
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'''MECDP synthase''' or '''2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase''' (MECPS) participates in mevalonate-independent steroid biosynthesis. It converts 2-phospho-4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol (2P-CDME) to 2-C-methyl-D-erythritol 2, 4-cyclodiphosphate (MECP) and CMP. MECPS contains metal binding sites which are required for catalysis<ref>PMID:11786530</ref>.
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==3D structures of MECDP synthase==
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== Relevance ==
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MECPS is a potential antimalarial drug target<ref>PMID:12499535</ref>.
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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== Structural highlights ==
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{{#tree:id=OrganizedByTopic|openlevels=0|
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<scene name='52/526157/Cv/7'>MECPS active site is located in a cleft between 2 subunits</scene> and contains the <scene name='52/526157/Cv/8'>metal ions</scene><ref>PMID:11997478</ref>. Water molecules are shown as red spheres.
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*'''MECPS'''
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==3D structures of MECDP synthase==
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[[MECDP synthase 3D structures]]
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**[[1knk]] - EcMECPS + Mn – ''Escherichia coli''<br />
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**[[1u3p]] - EcMECPS + Zn<br />
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**[[3f0d]] - BpMECPS + Zn – ''Burkholderia pseudomallei''<br />
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**[[3f0e]] - BpMECPS + Mg + Zn<br />
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**[[1jn1]] - HiMECPS + Co – ''Haemophilus influenzae'' <br />
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**[[1vh8]] - HiMECPS <br />
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**[[1vha]] - HiMECPS + Mn<br />
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**[[1iv1]] - TtMECPS – ''Thermus thermophilus''<br />
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**[[3f6m]] - YpMECPS – ''Yersinia pestis''<br />
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**[[1iv3]] - TtMECPS (mutant) + Mg<br />
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**[[3ghz]] - MECPS + Mg – ''Salmonella typhimurium''<br />
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**[[3re3]] - MECPS – ''Francisella tularensis''<br />
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**[[4c82]] - PfMECPS (mutant) + Zn – ''Plasmodium falciparum''<br />
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*MECPS binary complex
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**[[1gx1]] - EcMECPS + CDP + Mn + Zn<br />
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**[[1u3l]] - EcMECPS + CDP + Mg + Zn<br />
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**[[3f0f]] - BpMECPS + CMP + Mg + Zn<br />
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**[[3ike]] - BpMECPS + cytosine + Mg + Zn<br />
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**[[3k2x]], [[3ke1]] - BpMECPS + cytosine derivative + Zn<br />
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**[[3ikf]], [[3k14]], [[3q8h]] - BpMECPS + imidazo-thiazol derivative + Zn<br />
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**[[3f0g]], [[3ieq]] - BpMECPS + CMP + Zn<br />
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**[[2uzh]] - MECPS + CDP + Zn – ''Mycobacterium smegmatis''<br />
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**[[4c81]] - PfMECPS (mutant) + CDP + Zn <br />
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**[[3fpi]] - YpMECPS + CTP + Mg<br />
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**[[1iv2]] - TtMECPS (mutant) + CDP + Mg<br />
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**[[1iv4]] - TtMECPS (mutant) + CMP + Mg<br />
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**[[1h47]] - EcMECPS + CMP + Zn<br />
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**[[3jvh]] - BpMECPS + pyrimidine derivative + Zn<br />
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**[[1u40]] - EcMECPS + CDME + Zn<br />
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**[[1u43]] - EcMECPS + 2P-CDME<br />
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**[[1t0a]] - MECPS + farnesyl phosphate + Co + Zn - ''Shewanella oneidensis''<br />
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**[[2pmp]] - MECPS + CMP + Zn – ''Arabidopsis thaliana''<br />
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**[[4c8e]], [[4c8g]] - BcMECPS + CMP + Zn – ''Burkholderia cenocepacia''<br />
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**[[4c8i]] - BcMECPS + citrate + Zn <br />
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*MECPS ternary complex
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**[[3iew]] - BpMECPS + CDP + CTP + Zn<br />
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**[[1jy8]] – EcMECPS + MECP + CMP + Zn<br />
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**[[1knj]] - EcMECPS + MECP + CMP + Mn<br />
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**[[3mbm]] - BpMECPS + imidazo-thiazol derivative +cytosine + Zn<br />
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**[[3p10]] - BpMECPS + pyrimidine derivative + cytidine + Zn<br />
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**[[3t80]] - MECPS + pyrimidine derivative + cytidine + Zn – ''Salmonella enterica''<br />
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**[[1yqn]] – EcMECPS (mutant) + CMP + geranyl diphosphate + Mn + Zn<br />
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**[[2amt]] - EcMECPS + CMP derivative + geranyl diphosphate + Zn<br />
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**[[3elc]], [[3eor]], [[3ern]], [[3fba]] - EcMECPS + pyrimidine derivative + geranyl diphosphate + Zn<br />
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**[[3esj]] - EcMECPS + pyrimidine derivative + geranyl diphosphate + Mg + Zn<br />
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**[[2gzl]] - EcMECPS + inhibitor + geranyl diphosphate + Zn<br />
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*MECPS quaternary complex
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</StructureSection>
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**[[3p0z]], [[3qhd]] - BpMECPS + imidazo derivative + pyrimidine derivative + cytidine + Zn<br />
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== References ==
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**[[1h48]] - EcMECPS + MECP + CMP + geranyl diphosphate + Mn + Zn<br />
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<references/>
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}}
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[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Se-Met MECDP synthase trimer complex with CDP, sulfate, Mn+2 (purple) and Zn+2 (grey) ion, 1gx1

Drag the structure with the mouse to rotate

References

  1. ↑ Richard SB, Ferrer JL, Bowman ME, Lillo AM, Tetzlaff CN, Cane DE, Noel JP. Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway. J Biol Chem. 2002 Mar 8;277(10):8667-72. Epub 2002 Jan 10. PMID:11786530 doi:http://dx.doi.org/10.1074/jbc.C100739200
  2. ↑ Kishida H, Wada T, Unzai S, Kuzuyama T, Takagi M, Terada T, Shirouzu M, Yokoyama S, Tame JR, Park SY. Structure and catalytic mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) synthase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis. Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):23-31. Epub 2002, Dec 19. PMID:12499535
  3. ↑ Kemp LE, Bond CS, Hunter WN. Structure of 2C-methyl-D-erythritol 2,4- cyclodiphosphate synthase: an essential enzyme for isoprenoid biosynthesis and target for antimicrobial drug development. Proc Natl Acad Sci U S A. 2002 May 14;99(10):6591-6. Epub 2002 May 7. PMID:11997478 doi:10.1073/pnas.102679799

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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