Nitrate reductase

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{{STRUCTURE_2jim| PDB=2jim | SIZE=400| SCENE= |right|CAPTION=Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo (green) and molybdepterin guanine nucleotide (stick model), [[2jim]] }}
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<StructureSection load='' size='350' side='right' caption='Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry [[2jiq]])' scene='49/490062/Cv/3'>
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== Function ==
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'''Nitrate reductase''' (NR) catalyzes the reduction of NO<sub>3</sub> to NO<sub>2</sub> using NADPH<ref>PMID:13152110</ref>. NR active site contains Mo atom. Four types of NR are known:<br />
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'''eukaryotic assimilatory NR'''<br />
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'''bacterial cytoplasmic assimilatory''' (NAC)<br />
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'''bacterial membrane-bound respiratory''' (NAR)<br />
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'''Nitrate reductase''' (NR) catalyzes the reduction of NO3 to NO2 using NADPH . NR active site contains Mo atom. Four types of NR are known: one eukaryotic assimilatory NR and 3 bacterial NRs – cytoplasmic assimilatory (NAC), membrane-bound respiratory (NAR) and periplasmic dissimilatory (NAP). The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
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'''bacterial periplasmic dissimilatory''' (NAP).<br />
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The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
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== Structural highlights ==
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<scene name='49/490062/Cv/7'>Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3 and 2 molybdepterin guanine nucleotides</scene>. NAP active site includes a <scene name='49/490062/Cv/8'>six-coordinated Mo atom which coordinates to 2 MGD molecules and Cys140</scene>. The <scene name='49/490062/Cv/9'>NO3 molecule is guided to the active site by a funnel leading to the Mo atom</scene><ref>PMID:18327621</ref>.
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</StructureSection>
==3D structures of nitrate reductase==
==3D structures of nitrate reductase==
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**[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''<br />
**[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''<br />
**[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''<br />
**[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''<br />
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**[[3ml1]], [[3o5a]] - NAP catalytic subunit + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''
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**[[3ml1]], [[3o5a]] - NAP catalytic subunit + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''<br />
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**[[5yly]] – NR cytochrome b5 reductase domain + FAD – ''Ulva prolifera''<br />
*Bacterial respiratory NR
*Bacterial respiratory NR
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**[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''
**[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''
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*Eukaryotic NR
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**[[1cne]] – cNR + FAD – corn<br />
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**[[1cnf]], [[2cnd]] – cNR (mutant) + FAD + ADP<br />
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}}
}}
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry 2jiq)

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3D structures of nitrate reductase

Updated on 23-July-2019

References

  1. ↑ NICHOLAS DJ, NASON A. Molybdenum and nitrate reductase. II. Molybdenum as a constituent of nitrate reductase. J Biol Chem. 1954 Mar;207(1):353-60. PMID:13152110
  2. ↑ Najmudin S, Gonzalez PJ, Trincao J, Coelho C, Mukhopadhyay A, Cerqueira NM, Romao CC, Moura I, Moura JJ, Brondino CD, Romao MJ. Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum. J Biol Inorg Chem. 2008 Mar 8;. PMID:18327621 doi:10.1007/s00775-008-0359-6

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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