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4nul

From Proteopedia

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[[Image:4nul.gif|left|200px]]<br />
 
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<applet load="4nul" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="4nul, resolution 1.9&Aring;" />
 
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'''CLOSTRIDIUM BEIJERINCKII FLAVODOXIN MUTANT: D58P OXIDIZED'''<br />
 
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==Overview==
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==CLOSTRIDIUM BEIJERINCKII FLAVODOXIN MUTANT: D58P OXIDIZED==
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X-ray analyses of wild-type and mutant flavodoxins from Clostridium, beijerinckii show that the conformation of the peptide Gly57-Asp58, in a, bend near the isoalloxazine ring of FMN, is correlated with the oxidation, state of the FMN prosthetic group. The Gly-Asp peptide may adopt any of, three conformations: trans O-up, in which the carbonyl oxygen of Gly57, (O57) points toward the flavin ring; trans O-down, in which O57 points, away from the flavin; and cis O-down. Interconversions among these, conformers that are linked to oxidation-reduction of the flavin can, modulate the redox potentials of bound FMN. In the semiquinone and reduced, forms of the protein, the Gly57-Asp58 peptide adopts the trans O-up, conformation and accepts a hydrogen bond from the flavin N5H [Smith, W., W., ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9063874 (full description)]]
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<StructureSection load='4nul' size='340' side='right'caption='[[4nul]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4nul]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_beijerinckii Clostridium beijerinckii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NUL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nul OCA], [https://pdbe.org/4nul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nul RCSB], [https://www.ebi.ac.uk/pdbsum/4nul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nul ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FLAV_CLOBE FLAV_CLOBE] Low-potential electron donor to a number of redox enzymes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/4nul_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4nul ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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4NUL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_beijerinckii Clostridium beijerinckii]] with FMN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: FMN. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4NUL OCA]].
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*[[Flavodoxin 3D structures|Flavodoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Control of oxidation-reduction potentials in flavodoxin from Clostridium beijerinckii: the role of conformation changes., Ludwig ML, Pattridge KA, Metzger AL, Dixon MM, Eren M, Feng Y, Swenson RP, Biochemistry. 1997 Feb 11;36(6):1259-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9063874 9063874]
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[[Category: Clostridium beijerinckii]]
[[Category: Clostridium beijerinckii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dixon, M.M.]]
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[[Category: Dixon MM]]
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[[Category: Eren, M.]]
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[[Category: Eren M]]
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[[Category: Feng, Y.]]
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[[Category: Feng Y]]
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[[Category: Ludwig, M.L.]]
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[[Category: Ludwig ML]]
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[[Category: Metzger, A.L.]]
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[[Category: Metzger AL]]
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[[Category: Pattridge, K.A.]]
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[[Category: Pattridge KA]]
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[[Category: Swenson, R.]]
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[[Category: Swenson R]]
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[[Category: FMN]]
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[[Category: electron transport]]
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[[Category: flavoprotein]]
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[[Category: fmn]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:47:58 2007''
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Current revision

CLOSTRIDIUM BEIJERINCKII FLAVODOXIN MUTANT: D58P OXIDIZED

PDB ID 4nul

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