4r1r

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[[Image:4r1r.gif|left|200px]]<br />
 
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<applet load="4r1r" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="4r1r, resolution 3.2&Aring;" />
 
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'''RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI'''<br />
 
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==Overview==
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==RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI==
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BACKGROUND: Ribonucleotide reductase (RNR) is an essential enzyme in DNA, synthesis, catalyzing all de novo synthesis of deoxyribonucleotides. The, enzyme comprises two dimers, termed R1 and R2, and contains the redox, active cysteine residues, Cys462 and Cys225. The reduction of, ribonucleotides to deoxyribonucleotides involves the transfer of free, radicals. The pathway for the radical has previously been suggested from, crystallographic results, and is supported by site-directed mutagenesis, studies. Most RNRs are allosterically regulated through two different, nucleotide-binding sites: one site controls general activity and the other, controls substrate specificity. Our aim has been to crystallographically, demonstrate substrate binding and to locate the two effector-binding, sites. ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9309223 (full description)]]
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<StructureSection load='4r1r' size='340' side='right'caption='[[4r1r]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4r1r]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R1R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1r OCA], [https://pdbe.org/4r1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r1r RCSB], [https://www.ebi.ac.uk/pdbsum/4r1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIR1_ECOLI RIR1_ECOLI] Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide. It also provides redox-active cysteines.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/4r1r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4r1r ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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4R1R is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with TTP and GDP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1]]. Structure known Active Sites: ACA, ACB, ACG, SEE, SEI and SES. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4R1R OCA]].
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*[[Ribonucleotide reductase 3D structures|Ribonucleotide reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Binding of allosteric effectors to ribonucleotide reductase protein R1: reduction of active-site cysteines promotes substrate binding., Eriksson M, Uhlin U, Ramaswamy S, Ekberg M, Regnstrom K, Sjoberg BM, Eklund H, Structure. 1997 Aug 15;5(8):1077-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9309223 9309223]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Ribonucleoside-diphosphate reductase]]
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[[Category: Eklund H]]
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[[Category: Eklund, H.]]
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[[Category: Eriksson M]]
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[[Category: Eriksson, M.]]
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[[Category: GDP]]
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[[Category: TTP]]
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[[Category: allosteric regulation]]
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[[Category: complex (oxidoreductase/peptide)]]
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[[Category: deoxyribonucleotide synthesis]]
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[[Category: radical chemistry]]
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[[Category: ribonucleotide reductase]]
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[[Category: specificity]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:48:16 2007''
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Current revision

RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI

PDB ID 4r1r

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