1mft

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[[Image:1mft.gif|left|200px]]
 
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{{Structure
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==Crystal Structure Of Four-Helix Bundle Model==
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|PDB= 1mft |SIZE=350|CAPTION= <scene name='initialview01'>1mft</scene>, resolution 2.50&Aring;
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<StructureSection load='1mft' size='340' side='right'caption='[[1mft]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1mft]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MFT FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mft OCA], [https://pdbe.org/1mft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mft RCSB], [https://www.ebi.ac.uk/pdbsum/1mft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mft ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure Of Four-Helix Bundle Model'''
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__TOC__
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</StructureSection>
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==Overview==
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Although the analysis and design of turns that connect the strands in antiparallel beta-hairpins has reached an advanced state, much less is known concerning turns between antiparallel helices in helical hairpins. We have conducted an analysis of the structures and sequence preferences of two types of interhelical turns, each of which connects the two helices by a two-residue linker in an alphaL-beta conformation. Based on this analysis, it became apparent that the turn introduced into a designed four-helix bundle protein, DF1, did not occur within an optimal structural context. DF1 is a dimeric model for the diiron class of proteins. A longer loop with a beta-alphaR-beta conformation was inserted between two helices in the protein, and a sequence was chosen to stabilize its conformation. X-ray crystallography and NMR analysis of the protein showed the structure to be in excellent agreement with design.
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==About this Structure==
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1MFT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFT OCA].
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==Reference==
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Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713492 15713492]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: DeGrado, W.]]
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[[Category: DeGrado W]]
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[[Category: Geremia, S.]]
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[[Category: Geremia S]]
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[[Category: Kaplan, J.]]
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[[Category: Kaplan J]]
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[[Category: Lahr, S J.]]
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[[Category: Lahr SJ]]
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[[Category: North, B.]]
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[[Category: North B]]
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[[Category: Stayrook, S E.]]
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[[Category: Stayrook SE]]
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[[Category: ZN]]
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[[Category: alpha-helical bundle]]
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[[Category: helix turn helix]]
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[[Category: protein design]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:43:00 2008''
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Current revision

Crystal Structure Of Four-Helix Bundle Model

PDB ID 1mft

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