3j1o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:09, 21 February 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module==
==Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module==
-
<StructureSection load='3j1o' size='340' side='right' caption='[[3j1o]], [[Resolution|resolution]] 16.00&Aring;' scene=''>
+
<SX load='3j1o' size='340' side='right' viewer='molstar' caption='[[3j1o]], [[Resolution|resolution]] 16.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3j1o]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Gekko_coi Gekko coi] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J1O FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3j1o]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J1O FirstGlance]. <br>
-
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j1n|3j1n]]</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1o OCA], [https://pdbe.org/3j1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j1o RCSB], [https://www.ebi.ac.uk/pdbsum/3j1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j1o ProSAT]</span></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MED11, YMR112C, YM9718.11C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB4, MED17, YER022W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), MED8, YBR193C, YBR1403 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB6, MED22, YBR253W, YBR1721 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1118509 Gekko coi]), SRB5, MED18, YGR104C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB2, HRS2, MED20, YHR041C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), MED6, MTR32, YHR058C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
+
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j1o RCSB], [http://www.ebi.ac.uk/pdbsum/3j1o PDBsum]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/MED17_YEAST MED17_YEAST] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.<ref>PMID:11555651</ref> <ref>PMID:16076843</ref> <ref>PMID:16263706</ref> <ref>PMID:16885025</ref> <ref>PMID:9845373</ref>
-
Mediator, a large (21 polypeptides, MW approximately 1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function.
+
-
 
+
-
Interaction of the mediator head module with RNA polymerase II.,Cai G, Chaban YL, Imasaki T, Kovacs JA, Calero G, Penczek PA, Takagi Y, Asturias FJ Structure. 2012 May 9;20(5):899-910. doi: 10.1016/j.str.2012.02.023. PMID:22579255<ref>PMID:22579255</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
-
</StructureSection>
+
</SX>
-
[[Category: Gekko coi]]
+
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Asturias, F J]]
+
[[Category: Asturias FJ]]
-
[[Category: Imasaki, T]]
+
[[Category: Imasaki T]]
-
[[Category: Mediator head module]]
+
-
[[Category: Preinitiation complex]]
+
-
[[Category: Rna polymerase ii]]
+
-
[[Category: Transcription]]
+

Current revision

Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module

3j1o, resolution 16.00Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j1o"
Personal tools