1mnp

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[[Image:1mnp.gif|left|200px]]
 
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{{Structure
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==MANGANESE PEROXIDASE==
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|PDB= 1mnp |SIZE=350|CAPTION= <scene name='initialview01'>1mnp</scene>, resolution 2.0&Aring;
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<StructureSection load='1mnp' size='340' side='right'caption='[[1mnp]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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<table><tr><td colspan='2'>[[1mnp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MNP FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnp OCA], [https://pdbe.org/1mnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mnp RCSB], [https://www.ebi.ac.uk/pdbsum/1mnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PEM1_PHACH PEM1_PHACH] Catalyzes the oxidation of Mn(2+) to Mn(3+). The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin compounds.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mnp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mnp ConSurf].
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<div style="clear:both"></div>
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'''MANGANESE PEROXIDASE'''
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==See Also==
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*[[Manganese peroxidase|Manganese peroxidase]]
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__TOC__
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==Overview==
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</StructureSection>
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Manganese peroxidase from the white rot basidiomycete Phanerochaete chrysosporium has been crystallized in a form suitable for high-resolution X-ray structure determination. Crystals were grown from solutions containing 30% polyethylene glycol 8000, ammonium sulfate and cacodylate buffer at pH 6.5, using macroseeding techniques. A complete data set has been obtained to 2.06 A resolution. The data can be indexed in space group P1 with a = 45.96 A, b = 53.77 A, c = 84.87 A, alpha = 97.01 degrees, beta = 105.72 degrees and gamma = 90.1 degrees, with two peroxidase molecules per asymmetric unit, or in space group C2 with a = 163.23 A, b = 45.97 A, c = 53.72 A and beta = 97.16 degrees, with only one molecule in the assymetric unit. Lignin peroxidase, which shares about 57% sequence identity with manganese peroxidase, was used as a probe for molecular replacement. Unique rotation and translation solutions have been obtained in space groups P1 and C2. The structure has been partially refined in space group C2 to R = 0.22 for data between 10 and 2.06 A.
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[[Category: Large Structures]]
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[[Category: Phanerodontia chrysosporium]]
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==About this Structure==
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[[Category: Poulos TL]]
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1MNP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNP OCA].
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[[Category: Sundaramoorthy M]]
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==Reference==
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Preliminary crystallographic analysis of manganese peroxidase from Phanerochaete chrysosporium., Sundaramoorthy M, Kishi K, Gold MH, Poulos TL, J Mol Biol. 1994 May 20;238(5):845-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8182752 8182752]
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[[Category: Peroxidase]]
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[[Category: Phanerochaete chrysosporium]]
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[[Category: Single protein]]
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[[Category: Poulos, T L.]]
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[[Category: Sundaramoorthy, M.]]
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[[Category: CA]]
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[[Category: HEM]]
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[[Category: MN]]
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[[Category: heme peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:45:46 2008''
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Current revision

MANGANESE PEROXIDASE

PDB ID 1mnp

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