3l2x

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==Crystal Structure of Spin Labeled T4 Lysozyme Mutant 115-119RX==
==Crystal Structure of Spin Labeled T4 Lysozyme Mutant 115-119RX==
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<StructureSection load='3l2x' size='340' side='right' caption='[[3l2x]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='3l2x' size='340' side='right'caption='[[3l2x]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3l2x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L2X FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3l2x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L2X FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=RXR:[2,2,5,5-TETRAMETHYL-3,4-BIS(SULFANYLMETHYL)-2,5-DIHYDRO-1H-PYRROL-1-YL]OXIDANYL+RADICAL'>RXR</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">E, Lysozyme ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=RXR:[2,2,5,5-TETRAMETHYL-3,4-BIS(SULFANYLMETHYL)-2,5-DIHYDRO-1H-PYRROL-1-YL]OXIDANYL+RADICAL'>RXR</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2x OCA], [https://pdbe.org/3l2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l2x RCSB], [https://www.ebi.ac.uk/pdbsum/3l2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2x ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l2x RCSB], [http://www.ebi.ac.uk/pdbsum/3l2x PDBsum]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
==See Also==
==See Also==
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Enterobacteria phage t4]]
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[[Category: Escherichia virus T4]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
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[[Category: Cascio, D]]
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[[Category: Cascio D]]
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[[Category: Fleissner, M R]]
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[[Category: Fleissner MR]]
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[[Category: Hubbell, W L]]
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[[Category: Hubbell WL]]
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[[Category: Antimicrobial]]
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[[Category: Bacteriolytic enzyme]]
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[[Category: Epr]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Modified cysteine]]
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[[Category: Nitroxide spin label]]
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Current revision

Crystal Structure of Spin Labeled T4 Lysozyme Mutant 115-119RX

PDB ID 3l2x

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