3ll8

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==Crystal Structure of Calcineurin in Complex with AKAP79 Peptide==
==Crystal Structure of Calcineurin in Complex with AKAP79 Peptide==
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<StructureSection load='3ll8' size='340' side='right' caption='[[3ll8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='3ll8' size='340' side='right'caption='[[3ll8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ll8]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LL8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ll8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LL8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PPP3CA, CALNA, CNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), PPP3R1, CNA2, CNB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ll8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ll8 OCA], [https://pdbe.org/3ll8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ll8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ll8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ll8 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ll8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ll8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ll8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ll8 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/AKAP5_HUMAN AKAP5_HUMAN] May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.
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In hippocampal neurons, the scaffold protein AKAP79 recruits the phosphatase calcineurin to L-type Ca(2+) channels and couples Ca(2+) influx to activation of calcineurin and of its substrate, the transcription factor NFAT. Here we show that an IAIIIT anchoring site in human AKAP79 binds the same surface of calcineurin as the PxIxIT recognition peptide of NFAT, albeit more strongly. A modest decrease in calcineurin-AKAP affinity due to an altered anchoring sequence is compatible with NFAT activation, whereas a further decrease impairs activation. Counterintuitively, increasing calcineurin-AKAP affinity increases recruitment of calcineurin to the scaffold but impairs NFAT activation; this is probably due to both slower release of active calcineurin from the scaffold and sequestration of active calcineurin by 'decoy' AKAP sites. We propose that calcineurin-AKAP79 scaffolding promotes NFAT signaling by balancing strong recruitment of calcineurin with its efficient release to communicate with NFAT.
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Balanced interactions of calcineurin with AKAP79 regulate Ca(2+)-calcineurin-NFAT signaling.,Li H, Pink MD, Murphy JG, Stein A, Dell'acqua ML, Hogan PG Nat Struct Mol Biol. 2012 Feb 19;19(3):337-45. doi: 10.1038/nsmb.2238. PMID:22343722<ref>PMID:22343722</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Calcineurin|Calcineurin]]
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*[[Calcineurin 3D structures|Calcineurin 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphoprotein phosphatase]]
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[[Category: Large Structures]]
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[[Category: Hogan, P G]]
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[[Category: Hogan PG]]
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[[Category: Li, H]]
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[[Category: Li H]]
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[[Category: Akap79]]
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[[Category: Beta-augmentation]]
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[[Category: Calcineurin]]
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[[Category: Calmodulin-binding]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase-calcium binding protein complex]]
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[[Category: Iron]]
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[[Category: Lipoprotein]]
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[[Category: Membrane]]
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[[Category: Metal-binding]]
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[[Category: Myristate]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Protein phosphatase]]
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[[Category: Protein targeting]]
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[[Category: Protein-peptide docking]]
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Current revision

Crystal Structure of Calcineurin in Complex with AKAP79 Peptide

PDB ID 3ll8

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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