This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1n6x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:46, 10 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1n6x.jpg|left|200px]]
 
-
{{Structure
+
==RIP-phasing on Bovine Trypsin==
-
|PDB= 1n6x |SIZE=350|CAPTION= <scene name='initialview01'>1n6x</scene>, resolution 1.40&Aring;
+
<StructureSection load='1n6x' size='340' side='right'caption='[[1n6x]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ABN:BENZYLAMINE'>ABN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+
<table><tr><td colspan='2'>[[1n6x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6X FirstGlance]. <br>
-
|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABN:BENZYLAMINE'>ABN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6x OCA], [https://pdbe.org/1n6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6x RCSB], [https://www.ebi.ac.uk/pdbsum/1n6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6x ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n6/1n6x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n6x ConSurf].
 +
<div style="clear:both"></div>
-
'''RIP-phasing on Bovine Trypsin'''
+
==See Also==
-
 
+
*[[Trypsin 3D structures|Trypsin 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.
+
-
 
+
-
==About this Structure==
+
-
1N6X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6X OCA].
+
-
 
+
-
==Reference==
+
-
Specific radiation damage can be used to solve macromolecular crystal structures., Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S, Structure. 2003 Feb;11(2):217-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12575941 12575941]
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Trypsin]]
+
[[Category: Caffrey M]]
-
[[Category: Caffrey, M.]]
+
[[Category: Leiros H-KS]]
-
[[Category: Leiros, H K.S.]]
+
[[Category: McSweeney S]]
-
[[Category: McSweeney, S.]]
+
[[Category: Pan B]]
-
[[Category: Pan, B.]]
+
[[Category: Ravelli RBG]]
-
[[Category: Ravelli, R B.G.]]
+
-
[[Category: ABN]]
+
-
[[Category: CA]]
+
-
[[Category: GOL]]
+
-
[[Category: SO4]]
+
-
[[Category: hydrolase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:52:58 2008''
+

Current revision

RIP-phasing on Bovine Trypsin

PDB ID 1n6x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n6x"
Personal tools