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1n6y

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[[Image:1n6y.jpg|left|200px]]
 
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{{Structure
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==RIP-phasing on Bovine Trypsin==
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|PDB= 1n6y |SIZE=350|CAPTION= <scene name='initialview01'>1n6y</scene>, resolution 1.40&Aring;
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<StructureSection load='1n6y' size='340' side='right'caption='[[1n6y]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ABN:BENZYLAMINE'>ABN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[1n6y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6Y FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABN:BENZYLAMINE'>ABN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6y OCA], [https://pdbe.org/1n6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6y RCSB], [https://www.ebi.ac.uk/pdbsum/1n6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n6/1n6y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n6y ConSurf].
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<div style="clear:both"></div>
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'''RIP-phasing on Bovine Trypsin'''
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==See Also==
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.
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==About this Structure==
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1N6Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6Y OCA].
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==Reference==
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Specific radiation damage can be used to solve macromolecular crystal structures., Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S, Structure. 2003 Feb;11(2):217-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12575941 12575941]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Trypsin]]
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[[Category: Caffrey M]]
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[[Category: Caffrey, M.]]
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[[Category: Leiros H-KS]]
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[[Category: Leiros, H K.S]]
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[[Category: McSweeney S]]
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[[Category: McSweeney, S.]]
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[[Category: Pan B]]
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[[Category: Pan, B.]]
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[[Category: Ravelli RBG]]
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[[Category: Ravelli, R B.G.]]
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[[Category: ABN]]
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[[Category: CA]]
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[[Category: GOL]]
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[[Category: SO4]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:52:58 2008''
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Current revision

RIP-phasing on Bovine Trypsin

PDB ID 1n6y

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