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1n6j

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[[Image:1n6j.gif|left|200px]]
 
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{{Structure
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==Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2==
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|PDB= 1n6j |SIZE=350|CAPTION= <scene name='initialview01'>1n6j</scene>, resolution 2.20&Aring;
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<StructureSection load='1n6j' size='340' side='right'caption='[[1n6j]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1n6j]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6J FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= MEF2B OR XMEF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CABIN1 OR KIAA0330 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6j OCA], [https://pdbe.org/1n6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6j RCSB], [https://www.ebi.ac.uk/pdbsum/1n6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6j ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MEF2B_HUMAN MEF2B_HUMAN] Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n6/1n6j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n6j ConSurf].
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<div style="clear:both"></div>
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'''Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2'''
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==See Also==
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*[[Myocyte enhancer factor 2|Myocyte enhancer factor 2]]
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__TOC__
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==Overview==
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</StructureSection>
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The myocyte enhancer factor-2 (MEF2) family of transcription factors has important roles in the development and function of T cells, neuronal cells and muscle cells. MEF2 is capable of repressing or activating transcription by association with a variety of co-repressors or co-activators in a calcium-dependent manner. Transcriptional repression by MEF2 has attracted particular attention because of its potential role in hypertrophic responses of cardiomyocytes. Several MEF2 co-repressors, such as Cabin1/Cain and class II histone deacetylases (HDACs), have been identified. However, the molecular mechanism of their recruitment to specific promoters by MEF2 remains largely unknown. Here we report a crystal structure of the MADS-box/MEF2S domain of human MEF2B bound to a motif of the transcriptional co-repressor Cabin1 and DNA at 2.2 A resolution. The crystal structure reveals a stably folded MEF2S domain on the surface of the MADS box. Cabin1 adopts an amphipathic alpha-helix to bind a hydrophobic groove on the MEF2S domain, forming a triple-helical interaction. Our studies of the ternary Cabin1/MEF2/DNA complex show a general mechanism by which MEF2 recruits transcriptional co-repressor Cabin1 and class II HDACs to specific DNA sites.
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==About this Structure==
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1N6J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6J OCA].
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==Reference==
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Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2., Han A, Pan F, Stroud JC, Youn HD, Liu JO, Chen L, Nature. 2003 Apr 17;422(6933):730-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12700764 12700764]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Chen, L.]]
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[[Category: Chen L]]
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[[Category: Han, A.]]
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[[Category: Han A]]
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[[Category: Liu, J O.]]
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[[Category: Liu JO]]
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[[Category: Pan, F.]]
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[[Category: Pan F]]
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[[Category: Stroud, J C.]]
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[[Category: Stroud JC]]
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[[Category: Youn, H D.]]
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[[Category: Youn HD]]
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[[Category: histone deacetylase]]
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[[Category: mads-box]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:52:58 2008''
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Current revision

Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2

PDB ID 1n6j

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