This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3qpr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:48, 21 February 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==HK97 Prohead I encapsidating inactive virally encoded protease==
==HK97 Prohead I encapsidating inactive virally encoded protease==
-
<StructureSection load='3qpr' size='340' side='right' caption='[[3qpr]], [[Resolution|resolution]] 5.20&Aring;' scene=''>
+
<SX load='3qpr' size='340' side='right' viewer='molstar' caption='[[3qpr]], [[Resolution|resolution]] 5.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3qpr]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk97 Enterobacteria phage hk97]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3p8q 3p8q]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QPR FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3qpr]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3p8q 3p8q]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QPR FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3p8q|3p8q]], [[3e8k|3e8k]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.2&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37554 Enterobacteria phage HK97])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qpr OCA], [https://pdbe.org/3qpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qpr RCSB], [https://www.ebi.ac.uk/pdbsum/3qpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qpr ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qpr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qpr RCSB], [http://www.ebi.ac.uk/pdbsum/3qpr PDBsum]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
-
Virus capsid assembly requires recruiting and organizing multiple copies of protein subunits to form a closed shell for genome packaging that leads to infectivity. Many viruses encode scaffolding proteins to shift the equilibrium toward particle formation by promoting intersubunit interactions and stabilizing assembly intermediates. Bacteriophage HK97 lacks an explicit scaffolding protein, but the capsid protein (gp5) contains a scaffold-like N-terminal segment termed the delta domain. When gp5 is expressed in Escherichia coli, the delta domain guides 420 copies of the subunit into a procapsid with T=7 laevo icosahedral symmetry named Prohead-I. Prohead-I can be disassembled and reassembled under mild conditions and it cannot mature further. When the virally encoded protease (gp4) is coexpressed with gp5, it is incorporated into the capsid and digests the delta domain followed by autoproteolysis to produce the metastable Prohead-II. Prohead-I(+P) was isolated by coexpressing gp5 and an inactive mutant of gp4. Prohead-I and Prohead-I(+P) were compared by biochemical methods, revealing that the inactive protease stabilized the capsid against disassembly by chemical or physical stress. The crystal structure of Prohead-I(+P) was determined at 5.2 A resolution, and distortions were observed in the subunit tertiary structures similar to those observed previously in Prohead-II. Prohead-I(+P) differed from Prohead-II due to the presence of the delta domain and the resulting repositioning of the N-arms, explaining why Prohead-I can be reversibly dissociated and cannot mature. Low-resolution X-ray data enhanced the density of the relatively dynamic delta domains, revealing their quaternary arrangement and suggesting how they drive proper assembly.
+
-
 
+
-
The Prohead-I Structure of Bacteriophage HK97: Implications for Scaffold-Mediated Control of Particle Assembly and Maturation.,Huang RK, Khayat R, Lee KK, Gertsman I, Duda RL, Hendrix RW, Johnson JE J Mol Biol. 2011 Jan 27. PMID:21276801<ref>PMID:21276801</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
-
</StructureSection>
+
</SX>
-
[[Category: Enterobacteria phage hk97]]
+
[[Category: Escherichia virus HK97]]
-
[[Category: Duda, R L]]
+
[[Category: Large Structures]]
-
[[Category: Gertsman, I]]
+
[[Category: Duda RL]]
-
[[Category: Hendrix, R W]]
+
[[Category: Gertsman I]]
-
[[Category: Huang, R K]]
+
[[Category: Hendrix RW]]
-
[[Category: Johnson, J E]]
+
[[Category: Huang RK]]
-
[[Category: Khayat, R]]
+
[[Category: Johnson JE]]
-
[[Category: Lee, K K]]
+
[[Category: Khayat R]]
-
[[Category: Virus]]
+
[[Category: Lee KK]]
-
[[Category: Virus procapsid particle]]
+

Current revision

HK97 Prohead I encapsidating inactive virally encoded protease

3qpr, resolution 5.20Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3qpr"
Personal tools