3nvk

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==Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle==
==Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle==
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<StructureSection load='3nvk' size='340' side='right' caption='[[3nvk]], [[Resolution|resolution]] 3.21&Aring;' scene=''>
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<StructureSection load='3nvk' size='340' side='right'caption='[[3nvk]], [[Resolution|resolution]] 3.21&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3nvk]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NVK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3nvk]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NVK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.209&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF0060 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus]), rpl7ae, PF1367 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus]), flpA, PF0059 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nvk RCSB], [http://www.ebi.ac.uk/pdbsum/3nvk PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvk OCA], [https://pdbe.org/3nvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nvk RCSB], [https://www.ebi.ac.uk/pdbsum/3nvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nvk ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8U4M1_PYRFU Q8U4M1_PYRFU]
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Box C/D small nucleolar and Cajal body ribonucleoprotein particles (sno/scaRNPs) direct site-specific 2'-O-methylation of ribosomal and spliceosomal RNAs and are critical for gene expression. Here we report crystal structures of an archaeal box C/D RNP containing three core proteins (fibrillarin, Nop56/58, and L7Ae) and a half-mer box C/D guide RNA paired with a substrate RNA. The structure reveals a guide-substrate RNA duplex orientation imposed by a composite protein surface and the conserved GAEK motif of Nop56/58. Molecular modeling supports a dual C/D RNP structure that closely mimics that recently visualized by electron microscopy. The substrate-bound dual RNP model predicts an asymmetric protein distribution between the RNP that binds and methylates the substrate RNA. The predicted asymmetric nature of the holoenzyme is consistent with previous biochemical data on RNP assembly and provides a simple solution for accommodating base-pairing between the C/D guide RNA and large ribosomal and spliceosomal substrate RNAs.
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Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle.,Xue S, Wang R, Yang F, Terns RM, Terns MP, Zhang X, Maxwell ES, Li H Mol Cell. 2010 Sep 24;39(6):939-49. PMID:20864039<ref>PMID:20864039</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Ribosomal protein L7|Ribosomal protein L7]]
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*[[Kink-turn motif|Kink-turn motif]]
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== References ==
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*[[Ribosomal protein L7/L12|Ribosomal protein L7/L12]]
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<references/>
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*[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pyrococcus furiosus]]
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[[Category: Large Structures]]
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[[Category: Li, H]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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[[Category: Wang, R]]
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[[Category: Li H]]
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[[Category: Xue, S]]
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[[Category: Wang R]]
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[[Category: Nop domain kink turn methyl transferase]]
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[[Category: Xue S]]
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[[Category: Ribosome biogenesis spliceosome biogenesis]]
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[[Category: Transferase-rna complex]]
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Current revision

Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle

PDB ID 3nvk

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