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3rfu

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==Crystal structure of a copper-transporting PIB-type ATPase==
==Crystal structure of a copper-transporting PIB-type ATPase==
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<StructureSection load='3rfu' size='340' side='right' caption='[[3rfu]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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<StructureSection load='3rfu' size='340' side='right'caption='[[3rfu]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3rfu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RFU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RFU FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3rfu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RFU FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpg1024 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=91891 Legionella pneumophila subsp. pneumophila])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rfu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rfu RCSB], [http://www.ebi.ac.uk/pdbsum/3rfu PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rfu OCA], [https://pdbe.org/3rfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rfu RCSB], [https://www.ebi.ac.uk/pdbsum/3rfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rfu ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/COPA_LEGPH COPA_LEGPH] Couples the hydrolysis of ATP with the export of copper.<ref>PMID:24317491</ref> <ref>PMID:21716286</ref>
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Heavy-metal homeostasis and detoxification is crucial for cell viability. P-type ATPases of the class IB (PIB) are essential in these processes, actively extruding heavy metals from the cytoplasm of cells. Here we present the structure of a PIB-ATPase, a Legionella pneumophila CopA Cu(+)-ATPase, in a copper-free form, as determined by X-ray crystallography at 3.2 A resolution. The structure indicates a three-stage copper transport pathway involving several conserved residues. A PIB-specific transmembrane helix kinks at a double-glycine motif displaying an amphipathic helix that lines a putative copper entry point at the intracellular interface. Comparisons to Ca(2+)-ATPase suggest an ATPase-coupled copper release mechanism from the binding sites in the membrane via an extracellular exit site. The structure also provides a framework to analyse missense mutations in the human ATP7A and ATP7B proteins associated with Menkes' and Wilson's diseases.
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Crystal structure of a copper-transporting PIB-type ATPase.,Gourdon P, Liu XY, Skjorringe T, Morth JP, Moller LB, Pedersen BP, Nissen P Nature. 2011 Jun 29;475(7354):59-64. doi: 10.1038/nature10191. PMID:21716286<ref>PMID:21716286</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[ATPase|ATPase]]
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*[[ATPase 3D structures|ATPase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Legionella pneumophila subsp. pneumophila]]
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[[Category: Large Structures]]
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[[Category: Gourdon, P]]
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[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
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[[Category: Liu, X]]
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[[Category: Birk Moller L]]
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[[Category: Moller, L Birk]]
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[[Category: Gourdon P]]
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[[Category: Morth, J P]]
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[[Category: Liu X]]
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[[Category: Nissen, P]]
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[[Category: Morth JP]]
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[[Category: Pedersen, B Panyella]]
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[[Category: Nissen P]]
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[[Category: Skjorringe, T]]
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[[Category: Panyella Pedersen B]]
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[[Category: Alpha helical]]
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[[Category: Skjorringe T]]
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[[Category: Atp-binding]]
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[[Category: Copper transport]]
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[[Category: Cpc]]
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[[Category: Cu+]]
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[[Category: Cu+ exporting]]
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[[Category: Cxxc]]
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[[Category: Heavy-metal binding]]
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[[Category: Hydrolase]]
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[[Category: Ion transport]]
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[[Category: Magnesium]]
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[[Category: Membrane]]
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[[Category: Membrane protein]]
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[[Category: Metal-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: P-type atpase]]
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[[Category: Pi-atpase]]
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[[Category: Pib-atpase]]
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[[Category: Potassium]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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Current revision

Crystal structure of a copper-transporting PIB-type ATPase

PDB ID 3rfu

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