3u2w

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==Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese and glucose or a glucal species==
==Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese and glucose or a glucal species==
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<StructureSection load='3u2w' size='340' side='right' caption='[[3u2w]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
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<StructureSection load='3u2w' size='340' side='right'caption='[[3u2w]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3u2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U2W FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3u2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U2W FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=LCN:1,5-ANHYDRO-D-ARABINO-HEX-1-ENITOL'>LCN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qvb|3qvb]], [[3q4s|3q4s]], [[3rmw|3rmw]], [[3rmv|3rmv]], [[3t7m|3t7m]], [[3t7o|3t7o]], [[3t7n|3t7n]], [[3u2t|3u2t]], [[3u2u|3u2u]], [[3u2v|3u2v]], [[3u2x|3u2x]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LCN:1,5-ANHYDRO-D-ARABINO-HEX-1-ENITOL'>LCN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GYG, GYG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u2w OCA], [https://pdbe.org/3u2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u2w RCSB], [https://www.ebi.ac.uk/pdbsum/3u2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u2w ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycogenin_glucosyltransferase Glycogenin glucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.186 2.4.1.186] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u2w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u2w RCSB], [http://www.ebi.ac.uk/pdbsum/3u2w PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Disease ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/GLYG_HUMAN GLYG_HUMAN] Glycogen storage disease due to glycogenin deficiency. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
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Glycogenin initiates the synthesis of a maltosaccharide chain covalently attached to itself on Tyr195 via a stepwise glucosylation reaction, priming glycogen synthesis. We have captured crystallographic snapshots of human glycogenin during its reaction cycle, revealing a dynamic conformational switch between ground and active states mediated by the sugar donor UDP-glucose. This switch includes the ordering of a polypeptide stretch containing Tyr195, and major movement of an approximately 30-residue "lid" segment covering the active site. The rearranged lid guides the nascent maltosaccharide chain into the active site in either an intra- or intersubunit mode dependent upon chain length and steric factors and positions the donor and acceptor sugar groups for catalysis. The Thr83Met mutation, which causes glycogen storage disease XV, is conformationally locked in the ground state and catalytically inactive. Our data highlight the conformational plasticity of glycogenin and coexistence of two modes of glucosylation as integral to its catalytic mechanism.
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== Function ==
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[https://www.uniprot.org/uniprot/GLYG_HUMAN GLYG_HUMAN] Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.
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Conformational plasticity of glycogenin and its maltosaccharide substrate during glycogen biogenesis.,Chaikuad A, Froese DS, Berridge G, von Delft F, Oppermann U, Yue WW Proc Natl Acad Sci U S A. 2011 Dec 12. PMID:22160680<ref>PMID:22160680</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
*[[Glycogenin|Glycogenin]]
*[[Glycogenin|Glycogenin]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Glycogenin glucosyltransferase]]
 
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Arrowsmith, C H]]
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[[Category: Large Structures]]
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[[Category: Bountra, C]]
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[[Category: Arrowsmith CH]]
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[[Category: Chaikuad, A]]
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[[Category: Bountra C]]
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[[Category: Delft, F von]]
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[[Category: Chaikuad A]]
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[[Category: Edwards, A M]]
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[[Category: Edwards AM]]
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[[Category: Froese, D S]]
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[[Category: Froese DS]]
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[[Category: Krysztofinska, E]]
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[[Category: Krysztofinska E]]
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[[Category: Oppermann, U]]
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[[Category: Oppermann U]]
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[[Category: Structural genomic]]
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[[Category: Weigelt J]]
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[[Category: Weigelt, J]]
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[[Category: Yue WW]]
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[[Category: Yue, W W]]
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[[Category: Von Delft F]]
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[[Category: Glucal]]
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[[Category: Glycogen biosynthesis]]
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[[Category: Glycosylation]]
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[[Category: Glycosyltransferase]]
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[[Category: Sgc]]
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[[Category: Transferase]]
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Current revision

Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese and glucose or a glucal species

PDB ID 3u2w

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