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| | ==Moonlighting functions of FeoC in the regulation of ferrous iron transport in Feo== | | ==Moonlighting functions of FeoC in the regulation of ferrous iron transport in Feo== |
| - | <StructureSection load='4awx' size='340' side='right' caption='[[4awx]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4awx' size='340' side='right'caption='[[4awx]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4awx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AWX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4awx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AWX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wia|2wia]], [[2wib|2wib]], [[2wic|2wic]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4awx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4awx RCSB], [http://www.ebi.ac.uk/pdbsum/4awx PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4awx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awx OCA], [https://pdbe.org/4awx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4awx RCSB], [https://www.ebi.ac.uk/pdbsum/4awx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4awx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/FEOC_KLEP3 FEOC_KLEP3] May function as a transcriptional regulator that controls feoABC expression.[HAMAP-Rule:MF_01586] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4awx" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Klebsiella pneumoniae]] | | [[Category: Klebsiella pneumoniae]] |
| - | [[Category: Hsiao, C D]] | + | [[Category: Large Structures]] |
| - | [[Category: Hsu, Y L]] | + | [[Category: Hsiao CD]] |
| - | [[Category: Huang, T H]] | + | [[Category: Hsu Y-L]] |
| - | [[Category: Hung, K W]] | + | [[Category: Huang T-H]] |
| - | [[Category: Juan, T H]] | + | [[Category: Hung K-W]] |
| - | [[Category: Tsai, J Y]] | + | [[Category: Juan T-H]] |
| - | [[Category: Metal transport]]
| + | [[Category: Tsai J-Y]] |
| Structural highlights
Function
FEOC_KLEP3 May function as a transcriptional regulator that controls feoABC expression.[HAMAP-Rule:MF_01586]
Publication Abstract from PubMed
Feo is a transport system commonly used by bacteria to acquire environmental Fe(2+). It consists of three proteins: FeoA, FeoB, and FeoC. FeoB is a large protein with a cytosolic N-terminal domain (NFeoB) that contains a regulatory G protein domain and a helical S domain. The C-terminal region of FeoB is a transmembrane domain that likely acts as the Fe(2+) permease. NFeoB has been shown to form a trimer pore that may function as an Fe(2+) gate. FeoC is a small winged-helix protein that possesses four conserved cysteine residues with a consensus sequence that likely provides binding sites for the [Fe-S] cluster. Therefore, FeoC is presumed to be an [Fe-S] cluster-dependent regulator that directly controls transcription of the feo operon. Despite the apparent significance of the Feo system, however, the function of FeoC has not been experimentally demonstrated. Here, we show that Klebsiella pneumoniae FeoC (KpFeoC) forms a tight complex with the intracellular N-terminal domain of FeoB (KpNFeoB). The crystal structure of the complex reveals that KpFeoC binds to KpNFeoB between the switch II region of the G protein domain and the effector S domain and that the long KpFeoC W1 loop lies above the KpNFeoB nucleotide-binding site. These interactions suggest that KpFeoC modulates the guanine nucleotide-mediated signal transduction process. Moreover, we showed that binding of KpFeoC disrupts pore formation by interfering with KpNFeoB trimerization. These results provide strong evidence suggesting that KpFeoC plays a crucial role in regulating Fe(2+) transport in Klebsiella pneumonia in addition to the presumed gene regulator role.
Crystal structure of the Klebsiella pneumoniae NFeoB/FeoC complex and roles of FeoC in regulation of Fe2+ transport by the bacterial Feo system.,Hung KW, Tsai JY, Juan TH, Hsu YL, Hsiao CD, Huang TH J Bacteriol. 2012 Dec;194(23):6518-26. doi: 10.1128/JB.01228-12. Epub 2012 Sep, 28. PMID:23024345[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hung KW, Tsai JY, Juan TH, Hsu YL, Hsiao CD, Huang TH. Crystal structure of the Klebsiella pneumoniae NFeoB/FeoC complex and roles of FeoC in regulation of Fe2+ transport by the bacterial Feo system. J Bacteriol. 2012 Dec;194(23):6518-26. doi: 10.1128/JB.01228-12. Epub 2012 Sep, 28. PMID:23024345 doi:http://dx.doi.org/10.1128/JB.01228-12
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