1npm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:05, 30 October 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1npm.gif|left|200px]]
 
-
{{Structure
+
==NEUROPSIN, A SERINE PROTEASE EXPRESSED IN THE LIMBIC SYSTEM OF MOUSE BRAIN==
-
|PDB= 1npm |SIZE=350|CAPTION= <scene name='initialview01'>1npm</scene>, resolution 2.1&Aring;
+
<StructureSection load='1npm' size='340' side='right'caption='[[1npm]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=ACA:Catalytic+Triad+(Active+Site)'>ACA</scene> and <scene name='pdbsite=ACB:Catalytic+Triad+(Active+Site)'>ACB</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
+
<table><tr><td colspan='2'>[[1npm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NPM FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1npm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1npm OCA], [https://pdbe.org/1npm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1npm RCSB], [https://www.ebi.ac.uk/pdbsum/1npm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1npm ProSAT]</span></td></tr>
-
 
+
</table>
-
'''NEUROPSIN, A SERINE PROTEASE EXPRESSED IN THE LIMBIC SYSTEM OF MOUSE BRAIN'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/KLK8_MOUSE KLK8_MOUSE] Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.<ref>PMID:9556608</ref> <ref>PMID:10762375</ref> <ref>PMID:11880192</ref> <ref>PMID:12944500</ref> <ref>PMID:16537644</ref> <ref>PMID:16308352</ref> <ref>PMID:17182622</ref> <ref>PMID:17629414</ref>
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/1npm_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1npm ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
Neuropsin is a novel serine protease, the expression of which is highly localized in the limbic areas of the mouse brain and which is suggested to be involved in kindling epileptogenesis and hippocampal plasticity. The 2.1-A resolution crystal structure of neuropsin provides the first three-dimensional view of one of the serine proteases highly expressed in the nervous system, and reveals a serine protease fold that exhibits chimeric features between trypsin and nerve growth factor-gamma (NGFgamma), a member of the kallikrein family. Neuropsin possesses an N-glycosylated "kallikrein loop" but forms six disulfide bonds corresponding to those of trypsin. The ordered kallikrein loop projects proline toward the active site to restrict smaller residues or proline at the P2 position of substrates. Loop F, which participates in forming the S3/S4 sites, is similar to trypsin rather than NGFgamma. The unique conformations of loops G and H form an S1 pocket specific for both arginine and lysine. These characteristic loop structures forming the substrate-binding site suggest the novel substrate specificity of neuropsin and give a clue to the design of its specific inhibitors.
Neuropsin is a novel serine protease, the expression of which is highly localized in the limbic areas of the mouse brain and which is suggested to be involved in kindling epileptogenesis and hippocampal plasticity. The 2.1-A resolution crystal structure of neuropsin provides the first three-dimensional view of one of the serine proteases highly expressed in the nervous system, and reveals a serine protease fold that exhibits chimeric features between trypsin and nerve growth factor-gamma (NGFgamma), a member of the kallikrein family. Neuropsin possesses an N-glycosylated "kallikrein loop" but forms six disulfide bonds corresponding to those of trypsin. The ordered kallikrein loop projects proline toward the active site to restrict smaller residues or proline at the P2 position of substrates. Loop F, which participates in forming the S3/S4 sites, is similar to trypsin rather than NGFgamma. The unique conformations of loops G and H form an S1 pocket specific for both arginine and lysine. These characteristic loop structures forming the substrate-binding site suggest the novel substrate specificity of neuropsin and give a clue to the design of its specific inhibitors.
-
==About this Structure==
+
Crystal structure of neuropsin, a hippocampal protease involved in kindling epileptogenesis.,Kishi T, Kato M, Shimizu T, Kato K, Matsumoto K, Yoshida S, Shiosaka S, Hakoshima T J Biol Chem. 1999 Feb 12;274(7):4220-4. PMID:9933620<ref>PMID:9933620</ref>
-
1NPM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPM OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure of neuropsin, a hippocampal protease involved in kindling epileptogenesis., Kishi T, Kato M, Shimizu T, Kato K, Matsumoto K, Yoshida S, Shiosaka S, Hakoshima T, J Biol Chem. 1999 Feb 12;274(7):4220-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9933620 9933620]
+
</div>
 +
<div class="pdbe-citations 1npm" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Single protein]]
+
[[Category: Hakoshima T]]
-
[[Category: Hakoshima, T.]]
+
[[Category: Kato K]]
-
[[Category: Kato, K.]]
+
[[Category: Kato M]]
-
[[Category: Kato, M.]]
+
[[Category: Kishi T]]
-
[[Category: Kishi, T.]]
+
[[Category: Matsumoto K]]
-
[[Category: Matsumoto, K.]]
+
[[Category: Shimizu T]]
-
[[Category: Shimizu, T.]]
+
[[Category: Shiosaka S]]
-
[[Category: Shiosaka, S.]]
+
[[Category: Yoshida S]]
-
[[Category: Yoshida, S.]]
+
-
[[Category: NAG]]
+
-
[[Category: glycoprotein]]
+
-
[[Category: serine proteinase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:59:59 2008''
+

Current revision

NEUROPSIN, A SERINE PROTEASE EXPRESSED IN THE LIMBIC SYSTEM OF MOUSE BRAIN

PDB ID 1npm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1npm"
Personal tools