This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4fxq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:23, 1 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Full-length Certhrax toxin from Bacillus cereus in complex with Inhibitor P6==
==Full-length Certhrax toxin from Bacillus cereus in complex with Inhibitor P6==
-
<StructureSection load='4fxq' size='340' side='right' caption='[[4fxq]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
+
<StructureSection load='4fxq' size='340' side='right'caption='[[4fxq]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4fxq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FXQ FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4fxq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FXQ FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G9L:8-FLUORO-2-(3-PIPERIDIN-1-YLPROPANOYL)-1,3,4,5-TETRAHYDROBENZO[C][1,6]NAPHTHYRIDIN-6(2H)-ONE'>G9L</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9599&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Amino acids 18-471, BCE_G9241_pBC218_0027, pBC218_0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G9L:8-FLUORO-2-(3-PIPERIDIN-1-YLPROPANOYL)-1,3,4,5-TETRAHYDROBENZO[C][1,6]NAPHTHYRIDIN-6(2H)-ONE'>G9L</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fxq RCSB], [http://www.ebi.ac.uk/pdbsum/4fxq PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fxq OCA], [https://pdbe.org/4fxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fxq RCSB], [https://www.ebi.ac.uk/pdbsum/4fxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fxq ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/CRAX_BACCE CRAX_BACCE] A probable mono(ADP-ribosyl)transferase; it is not known which residue is targeted for ADP-ribosylation. Upon expression in yeast cells causes cell death.<ref>PMID:21170356</ref>
-
We identified Certhrax, the first anthrax-like mART toxin from the pathogenic G9241 strain of Bacillus cereus. Certhrax shares 31% sequence identity with anthrax lethal factor from Bacillus anthracis, however, we have shown that the toxicity of Certhrax resides in the mART domain, while anthrax uses a metalloprotease mechanism. Like anthrax lethal factor, Certhrax was found to require protective antigen for host cell entry. This two-domain enzyme was shown to be 60-fold more toxic to mammalian cells than anthrax lethal factor. Certhrax localizes to distinct regions within mouse RAW264.7 cells by 10 min post-infection and is extranuclear in its cellular location. Substitution of catalytic residues shows that the mART function is responsible for the toxicity, and it binds NAD+ with high affinity (KD = 52.3 +/- 12.2 muM). We report the 2.2 A Certhrax structure, highlighting its structural similarities and differences with anthrax lethal factor. We also determined the crystal structures of two good inhibitors (P6, KD = 1.7 +/- 0.2 muM, Ki = 1.8 +/- 0.4 muM; and PJ34, KD = 5.8 +/- 2.6 muM, Ki = 9.6 +/- 0.3 muM) in complex with Certhrax. As with other toxins in this family, the phosphate-nicotinamide loop moves toward the NAD+ binding site with bound inhibitor. These results indicate that Certhrax may be important in the pathogenesis of B. cereus.
+
-
 
+
-
Certhrax toxin, an Anthrax-related ADP-ribosyltransferase from Bacillus cereus.,Visschedyk D, Rochon A, Tempel W, Dimov S, Park HW, Merrill AR J Biol Chem. 2012 Sep 19. PMID:22992735<ref>PMID:22992735</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
== References ==
== References ==
<references/>
<references/>
Line 20: Line 15:
</StructureSection>
</StructureSection>
[[Category: Bacillus cereus]]
[[Category: Bacillus cereus]]
-
[[Category: Dimov, S]]
+
[[Category: Large Structures]]
-
[[Category: Kimber, M S]]
+
[[Category: Dimov S]]
-
[[Category: Merrill, A R]]
+
[[Category: Kimber MS]]
-
[[Category: Park, H W]]
+
[[Category: Merrill AR]]
-
[[Category: Visschedyk, D D]]
+
[[Category: Park HW]]
-
[[Category: Bacterial toxin]]
+
[[Category: Visschedyk DD]]
-
[[Category: Mixed alpha/beta]]
+
-
[[Category: Mono-adp-ribosyltransferase]]
+
-
[[Category: Toxin]]
+
-
[[Category: Transferase-transferase inhibitor complex]]
+

Current revision

Full-length Certhrax toxin from Bacillus cereus in complex with Inhibitor P6

PDB ID 4fxq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4fxq"
Personal tools