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4fgv

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==Crystal structure of free CRM1 (crystal form 1)==
==Crystal structure of free CRM1 (crystal form 1)==
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<StructureSection load='4fgv' size='340' side='right' caption='[[4fgv]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
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<StructureSection load='4fgv' size='340' side='right'caption='[[4fgv]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4fgv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_dsm_1495 Chaetomium thermophilum var. thermophilum dsm 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FGV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4fgv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FGV FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fgv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fgv RCSB], [http://www.ebi.ac.uk/pdbsum/4fgv PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.941&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fgv OCA], [https://pdbe.org/4fgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fgv RCSB], [https://www.ebi.ac.uk/pdbsum/4fgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fgv ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/G0RZB7_CHATD G0RZB7_CHATD]
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In eukaryotes, the nucleocytoplasmic transport of macromolecules is mainly mediated by soluble nuclear transport receptors of the karyopherin-beta superfamily termed importins and exportins. The highly versatile exportin chromosome region maintenance 1 (CRM1) is essential for nuclear depletion of numerous structurally and functionally unrelated protein and ribonucleoprotein cargoes. CRM1 has been shown to adopt a toroidal structure in several functional transport complexes and was thought to maintain this conformation throughout the entire nucleocytoplasmic transport cycle. We solved crystal structures of free CRM1 from the thermophilic eukaryote Chaetomium thermophilum. Surprisingly, unbound CRM1 exhibits an overall extended and pitched superhelical conformation. The two regulatory regions, namely the acidic loop and the C-terminal alpha-helix, are dramatically repositioned in free CRM1 in comparison with the ternary CRM1-Ran-Snurportin1 export complex. Single-particle EM analysis demonstrates that, in a noncrystalline environment, free CRM1 exists in equilibrium between extended, superhelical and compact, ring-like conformations. Molecular dynamics simulations show that the C-terminal helix plays an important role in regulating the transition from an extended to a compact conformation and reveal how the binding site for nuclear export signals of cargoes is modulated by different CRM1 conformations. Combining these results, we propose a model for the cooperativity of CRM1 export complex assembly involving the long-range allosteric communication between the distant binding sites of GTP-bound Ran and cargo.
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Structural basis for cooperativity of CRM1 export complex formation.,Monecke T, Haselbach D, Voss B, Russek A, Neumann P, Thomson E, Hurt E, Zachariae U, Stark H, Grubmuller H, Dickmanns A, Ficner R Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):960-5. doi: 10.1073/pnas.1215214110., Epub 2012 Dec 31. PMID:23277578<ref>PMID:23277578</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Exportin 3D structures|Exportin 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Chaetomium thermophilum var. thermophilum dsm 1495]]
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[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
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[[Category: Dickmanns, A]]
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[[Category: Large Structures]]
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[[Category: Ficner, R]]
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[[Category: Dickmanns A]]
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[[Category: Monecke, T]]
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[[Category: Ficner R]]
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[[Category: Neumann, P]]
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[[Category: Monecke T]]
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[[Category: Heat repeat protein]]
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[[Category: Neumann P]]
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[[Category: Importin-beta superfamily]]
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[[Category: Nuclear export of numerous protein and rnp cargo]]
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[[Category: Transport protein]]
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Current revision

Crystal structure of free CRM1 (crystal form 1)

PDB ID 4fgv

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