|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==Crystal structure of type effector Tse1 from Pseudomonas aeruginousa== | | ==Crystal structure of type effector Tse1 from Pseudomonas aeruginousa== |
| - | <StructureSection load='4f0v' size='340' side='right' caption='[[4f0v]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='4f0v' size='340' side='right'caption='[[4f0v]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f0v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F0V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f0v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F0V FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e0w|4e0w]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0v OCA], [https://pdbe.org/4f0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f0v RCSB], [https://www.ebi.ac.uk/pdbsum/4f0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f0v ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA1844 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f0v RCSB], [http://www.ebi.ac.uk/pdbsum/4f0v PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TSE1_PSEAE TSE1_PSEAE] Toxin secreted by the H1 type VI (H1-T6SS) secretion system into the periplasm of recipient cells. Degrades peptidoglycan via amidase activity thereby helping itself to compete with other bacteria (PubMed:21776080, PubMed:22931054, PubMed:22813741, PubMed:22700987). To protect itself, the bacterium synthesizes immunity protein Tsi1 that specifically interacts with and inactivates cognate toxin (PubMed:21776080, PubMed:22931054, PubMed:22700987).<ref>PMID:21776080</ref> <ref>PMID:22700987</ref> <ref>PMID:22813741</ref> <ref>PMID:22931054</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 16: |
Line 17: |
| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4f0v" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseudomonas aeruginosa pao1]] | + | [[Category: Large Structures]] |
| - | [[Category: Dong, Y H]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Gao, Z Q]] | + | [[Category: Dong YH]] |
| - | [[Category: Zhang, H]] | + | [[Category: Gao ZQ]] |
| - | [[Category: Hydrolase]] | + | [[Category: Zhang H]] |
| - | [[Category: Nlpc/p60 domain]]
| + | |
| - | [[Category: Type vi amidase effector]]
| + | |
| Structural highlights
Function
TSE1_PSEAE Toxin secreted by the H1 type VI (H1-T6SS) secretion system into the periplasm of recipient cells. Degrades peptidoglycan via amidase activity thereby helping itself to compete with other bacteria (PubMed:21776080, PubMed:22931054, PubMed:22813741, PubMed:22700987). To protect itself, the bacterium synthesizes immunity protein Tsi1 that specifically interacts with and inactivates cognate toxin (PubMed:21776080, PubMed:22931054, PubMed:22700987).[1] [2] [3] [4]
Publication Abstract from PubMed
The type VI secretion systems (T6SS) have emerging roles in interspecies competition. In order to have an advantage in defense against other organisms, this system in Pseudomonas aeruginosa delivers a peptidoglycan amidase (Tse1) to the periplasmic space of a competitor. An immune protein (Tsi1) is also produced by the bacterium to protect itself from damage caused by Tse1. Tsi1 directly interacts with Tse1. We report that the crystal structure of Tse1 displays a common CHAP protein fold. Strikingly, our structures showed that the third residue in the catalytic triad may be novel as this residue type has not been observed previously.
Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa.,Zhang H, Gao ZQ, Su XD, Dong YH FEBS Lett. 2012 Sep 21;586(19):3193-9. doi: 10.1016/j.febslet.2012.06.036. Epub, 2012 Jun 29. PMID:22750141[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD. Type VI secretion delivers bacteriolytic effectors to target cells. Nature. 2011 Jul 20;475(7356):343-7. doi: 10.1038/nature10244. PMID:21776080 doi:http://dx.doi.org/10.1038/nature10244
- ↑ Ding J, Wang W, Feng H, Zhang Y, Wang DC. Structural insights into the Pseudomonas aeruginosa type VI virulence effector Tse1 bacteriolysis and self-protection mechanisms. J Biol Chem. 2012 Jun 14. PMID:22700987 doi:10.1074/jbc.M112.368043
- ↑ Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD. Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system. Cell Rep. 2012 Jun 28;1(6):656-64. Epub 2012 May 31. PMID:22813741 doi:http://dx.doi.org/10.1016/j.celrep.2012.05.016
- ↑ Shang G, Liu X, Lu D, Zhang J, Li N, Zhu C, Liu S, Yu Q, Zhao Y, Zhang H, Hu J, Cang H, Xu S, Gu L. Structural insight into functioning of Pseudomonas aeruginosa peptidoglycan-hydolase Tse1 and its immunity protein Tsi1. Biochem J. 2012 Aug 29. PMID:22931054 doi:10.1042/BJ20120668
- ↑ Zhang H, Gao ZQ, Su XD, Dong YH. Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa. FEBS Lett. 2012 Sep 21;586(19):3193-9. doi: 10.1016/j.febslet.2012.06.036. Epub, 2012 Jun 29. PMID:22750141 doi:http://dx.doi.org/10.1016/j.febslet.2012.06.036
|
