4f49
From Proteopedia
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==2.25A resolution structure of Transmissible Gastroenteritis Virus Protease containing a covalently bound Dipeptidyl Inhibitor== | ==2.25A resolution structure of Transmissible Gastroenteritis Virus Protease containing a covalently bound Dipeptidyl Inhibitor== | ||
| - | <StructureSection load='4f49' size='340' side='right' caption='[[4f49]], [[Resolution|resolution]] 2.25Å' scene=''> | + | <StructureSection load='4f49' size='340' side='right'caption='[[4f49]], [[Resolution|resolution]] 2.25Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4f49]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[4f49]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_transmissible_gastroenteritis_coronavirus_strain_Purdue Porcine transmissible gastroenteritis coronavirus strain Purdue]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F49 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K36:(1S,2S)-2-({N-[(BENZYLOXY)CARBONYL]-L-LEUCYL}AMINO)-1-HYDROXY-3-[(3S)-2-OXOPYRROLIDIN-3-YL]PROPANE-1-SULFONIC+ACID'>K36</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K36:(1S,2S)-2-({N-[(BENZYLOXY)CARBONYL]-L-LEUCYL}AMINO)-1-HYDROXY-3-[(3S)-2-OXOPYRROLIDIN-3-YL]PROPANE-1-SULFONIC+ACID'>K36</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f49 OCA], [https://pdbe.org/4f49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f49 RCSB], [https://www.ebi.ac.uk/pdbsum/4f49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f49 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/R1A_CVPPU R1A_CVPPU] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
| + | <div class="pdbe-citations 4f49" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Porcine transmissible gastroenteritis coronavirus strain | + | [[Category: Large Structures]] |
| - | [[Category: Alliston | + | [[Category: Porcine transmissible gastroenteritis coronavirus strain Purdue]] |
| - | [[Category: Battaile | + | [[Category: Alliston KR]] |
| - | [[Category: Chang | + | [[Category: Battaile KP]] |
| - | [[Category: Groutas | + | [[Category: Chang K-O]] |
| - | [[Category: Kim | + | [[Category: Groutas WC]] |
| - | [[Category: Lovell | + | [[Category: Kim Y]] |
| - | [[Category: Mandadapu | + | [[Category: Lovell S]] |
| - | [[Category: Tiew | + | [[Category: Mandadapu SR]] |
| - | + | [[Category: Tiew K-C]] | |
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Current revision
2.25A resolution structure of Transmissible Gastroenteritis Virus Protease containing a covalently bound Dipeptidyl Inhibitor
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