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4hoq

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Crystal Structure of Full-Length Human IFIT5

Structural highlights

4hoq is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.07Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IFIT5_HUMAN Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and binds tRNAs.^[1] ^[2]

Publication Abstract from PubMed

Interferon-induced proteins with tetratricopeptide repeats (IFITs) are innate immune effector molecules that are thought to confer antiviral defence through disruption of protein-protein interactions in the host translation-initiation machinery. However, it was recently discovered that IFITs can directly recognize viral RNA bearing a 5'-triphosphate group (PPP-RNA), which is a molecular signature that distinguishes it from host RNA. Here we report crystal structures of human IFIT5, its complex with PPP-RNAs, and an amino-terminal fragment of IFIT1. The structures reveal a new helical domain that houses a positively charged cavity designed to specifically engage only single-stranded PPP-RNA, thus distinguishing it from the canonical cytosolic sensor of double-stranded viral PPP-RNA, retinoic acid-inducible gene I (RIG-I, also known as DDX58). Mutational analysis, proteolysis and gel-shift assays reveal that PPP-RNA is bound in a non-sequence-specific manner and requires a 5'-overhang of approximately three nucleotides. Abrogation of PPP-RNA binding in IFIT1 and IFIT5 was found to cause a defect in the antiviral response by human embryonic kidney cells. These results demonstrate the mechanism by which IFIT proteins selectively recognize viral RNA, and lend insight into their downstream effector function.

Structural basis for viral 5'-PPP-RNA recognition by human IFIT proteins.,Abbas YM, Pichlmair A, Gorna MW, Superti-Furga G, Nagar B Nature. 2013 Jan 13. doi: 10.1038/nature11783. PMID:23334420^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K. tRNA Binding, Structure, and Localization of the Human Interferon-Induced Protein IFIT5. Mol Cell. 2013 Feb 21;49(4):743-50. doi: 10.1016/j.molcel.2012.12.015. Epub 2013 , Jan 11. PMID:23317505 doi:http://dx.doi.org/10.1016/j.molcel.2012.12.015
↑ Abbas YM, Pichlmair A, Gorna MW, Superti-Furga G, Nagar B. Structural basis for viral 5'-PPP-RNA recognition by human IFIT proteins. Nature. 2013 Jan 13. doi: 10.1038/nature11783. PMID:23334420 doi:http://dx.doi.org/10.1038/nature11783
↑ Abbas YM, Pichlmair A, Gorna MW, Superti-Furga G, Nagar B. Structural basis for viral 5'-PPP-RNA recognition by human IFIT proteins. Nature. 2013 Jan 13. doi: 10.1038/nature11783. PMID:23334420 doi:http://dx.doi.org/10.1038/nature11783

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4hoq"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Full-Length Human IFIT5==
-<StructureSection load='4hoq' size='340' side='right' caption='[[4hoq]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
+<StructureSection load='4hoq' size='340' side='right'caption='[[4hoq]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4hoq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HOQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HOQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4hoq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HOQ FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hos|4hos]], [[4hot|4hot]], [[4hor|4hor]], [[4hou|4hou]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IFIT5, IFIT5/ISG58, ISG58, RI58 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hoq OCA], [https://pdbe.org/4hoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hoq RCSB], [https://www.ebi.ac.uk/pdbsum/4hoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hoq ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hoq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hoq RCSB], [http://www.ebi.ac.uk/pdbsum/4hoq PDBsum]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/IFIT5_HUMAN IFIT5_HUMAN] Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and binds tRNAs.<ref>PMID:23317505</ref> <ref>PMID:23334420</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4hoq" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 21: / Line 24: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Abbas, Y M]]
+[[Category: Large Structures]]
-[[Category: Gorna, M W]]
+[[Category: Abbas YM]]
-[[Category: Nagar, B]]
+[[Category: Gorna MW]]
-[[Category: Pichlmair, A]]
+[[Category: Nagar B]]
-[[Category: Superti-Furga, G]]
+[[Category: Pichlmair A]]
-[[Category: Antiviral]]
+[[Category: Superti-Furga G]]
-[[Category: Rna]]
-[[Category: Rna binding]]
-[[Category: Rna binding protein]]
-[[Category: Tpr]]

4hoq

From Proteopedia

Current revision

Crystal Structure of Full-Length Human IFIT5

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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