4gwq

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==Structure of the Mediator Head Module from S. cerevisiae in complex with the carboxy-terminal domain (CTD) of RNA Polymerase II Rpb1 subunit==
==Structure of the Mediator Head Module from S. cerevisiae in complex with the carboxy-terminal domain (CTD) of RNA Polymerase II Rpb1 subunit==
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<StructureSection load='4gwq' size='340' side='right' caption='[[4gwq]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
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<StructureSection load='4gwq' size='340' side='right'caption='[[4gwq]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gwq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast], [http://en.wikipedia.org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GWQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gwq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GWQ FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rj1|3rj1]], [[2hzm|2hzm]], [[2hzs|2hzs]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gwq OCA], [https://pdbe.org/4gwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gwq RCSB], [https://www.ebi.ac.uk/pdbsum/4gwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gwq ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gwq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gwq RCSB], [http://www.ebi.ac.uk/pdbsum/4gwq PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/MED11_YEAST MED11_YEAST] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex (PIC) with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. The essential MED11/22 heterodimer specifically functions in promoting stable PIC formation.<ref>PMID:16076843</ref> <ref>PMID:16263706</ref> <ref>PMID:16885025</ref> <ref>PMID:18691966</ref>
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The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests a basis for regulation by phosphorylation of the CTD. Biochemical studies show a requirement for Mediator-CTD interaction for transcription.
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Structure of the Mediator Head module bound to the carboxy-terminal domain of RNA polymerase II.,Robinson PJ, Bushnell DA, Trnka MJ, Burlingame AL, Kornberg RD Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17931-5. doi:, 10.1073/pnas.1215241109. Epub 2012 Oct 15. PMID:23071300<ref>PMID:23071300</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Baker's yeast]]
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[[Category: Large Structures]]
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[[Category: Clostridium acetobutylicum]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Burlingame AL]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Bushnell DA]]
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[[Category: Burlingame, A L]]
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[[Category: Kornberg RD]]
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[[Category: Bushnell, D A]]
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[[Category: Robinson PJJ]]
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[[Category: Kornberg, R D]]
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[[Category: Trnka MJ]]
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[[Category: Robinson, P J.J]]
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[[Category: Trnka, M J]]
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[[Category: Binding site]]
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[[Category: Mediator complex]]
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[[Category: Model]]
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[[Category: Molecular]]
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[[Category: Phosphorylation]]
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[[Category: Protein structure]]
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[[Category: Protein subunit]]
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[[Category: Rna polymerase ii]]
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[[Category: Structure-activity relationship]]
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[[Category: Tertiary]]
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[[Category: Transcription]]
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Current revision

Structure of the Mediator Head Module from S. cerevisiae in complex with the carboxy-terminal domain (CTD) of RNA Polymerase II Rpb1 subunit

PDB ID 4gwq

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