4geq

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Crystal structure of the Spc24-Spc25/Cnn1 binding interface

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 ==Crystal structure of the Spc24-Spc25/Cnn1 binding interface==
-<StructureSection load='4geq' size='340' side='right' caption='[[4geq]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
+<StructureSection load='4geq' size='340' side='right'caption='[[4geq]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c]), SPC24, YM9718.16C, YMR117C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [https://pdbe.org/4geq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [https://www.ebi.ac.uk/pdbsum/4geq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4geq ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref>
-The Ndc80 complex is the key microtubule-binding element of the kinetochore. In contrast to the well-characterized interaction of Ndc80-Nuf2 heads with microtubules, little is known about how the Spc24-25 heterodimer connects to centromeric chromatin. Here, we present molecular details of Spc24-25 in complex with the histone-fold protein Cnn1/CENP-T illustrating how this connection ultimately links microtubules to chromosomes. The conserved Ndc80 receptor motif of Cnn1 is bound as an alpha helix in a hydrophobic cleft at the interface between Spc24 and Spc25. Point mutations that disrupt the Ndc80-Cnn1 interaction also abrogate binding to the Mtw1 complex and are lethal in yeast. We identify a Cnn1-related motif in the Dsn1 subunit of the Mtw1 complex, necessary for Ndc80 binding and essential for yeast growth. Replacing this region with the Cnn1 peptide restores viability demonstrating functionality of the Ndc80-binding module in different molecular contexts. Finally, phosphorylation of the Cnn1 N-terminus coordinates the binding of the two competing Ndc80 interaction partners. Together, our data provide structural insights into the modular binding mechanism of the Ndc80 complex to its centromere recruiters.
-A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.,Malvezzi F, Litos G, Schleiffer A, Heuck A, Mechtler K, Clausen T, Westermann S EMBO J. 2013 Feb 6;32(3):409-23. doi: 10.1038/emboj.2012.356. Epub 2013 Jan 18. PMID:23334295<ref>PMID:23334295</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Saccharomyces cerevisiae s288c]]
+[[Category: Large Structures]]
-[[Category: Clausen, T]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Heuck, A]]
+[[Category: Clausen T]]
-[[Category: Litos, G]]
+[[Category: Heuck A]]
-[[Category: Malvezzi, F]]
+[[Category: Litos G]]
-[[Category: Schleiffer, A]]
+[[Category: Malvezzi F]]
-[[Category: Westermann, S]]
+[[Category: Schleiffer A]]
-[[Category: Cell cycle]]
+[[Category: Westermann S]]
-[[Category: Kinetochore component]]
-[[Category: Ndc80-binding motif]]
-[[Category: Nucleus]]
-[[Category: Protein-protein complex]]
-[[Category: Rwd domain]]

4geq

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Crystal structure of the Spc24-Spc25/Cnn1 binding interface

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Function

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