This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3j7i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:28, 20 March 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Structure of alpha- and beta- tubulin in GMPCPP-microtubules==
==Structure of alpha- and beta- tubulin in GMPCPP-microtubules==
-
<StructureSection load='3j7i' size='340' side='right' caption='[[3j7i]], [[Resolution|resolution]] 8.90&Aring;' scene=''>
+
<SX load='3j7i' size='340' side='right' viewer='molstar' caption='[[3j7i]], [[Resolution|resolution]] 8.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3j7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J7I FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3j7i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J7I FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.9&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j7i RCSB], [http://www.ebi.ac.uk/pdbsum/3j7i PDBsum]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7i OCA], [https://pdbe.org/3j7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j7i RCSB], [https://www.ebi.ac.uk/pdbsum/3j7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7i ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/TBA1A_PIG TBA1A_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
-
Microtubules are dynamic polymers that stochastically switch between growing and shrinking phases. Microtubule dynamics are regulated by guanosine triphosphate (GTP) hydrolysis by beta-tubulin, but the mechanism of this regulation remains elusive because high-resolution microtubule structures have only been revealed for the guanosine diphosphate (GDP) state. In this paper, we solved the cryoelectron microscopy (cryo-EM) structure of microtubule stabilized with a GTP analogue, guanylyl 5'-alpha,beta-methylenediphosphonate (GMPCPP), at 8.8-A resolution by developing a novel cryo-EM image reconstruction algorithm. In contrast to the crystal structures of GTP-bound tubulin relatives such as gamma-tubulin and bacterial tubulins, significant changes were detected between GMPCPP and GDP-taxol microtubules at the contacts between tubulins both along the protofilament and between neighboring protofilaments, contributing to the stability of the microtubule. These findings are consistent with the structural plasticity or lattice model and suggest the structural basis not only for the regulatory mechanism of microtubule dynamics but also for the recognition of the nucleotide state of the microtubule by several microtubule-binding proteins, such as EB1 or kinesin.
+
-
 
+
-
Conformational changes in tubulin in GMPCPP and GDP-taxol microtubules observed by cryoelectron microscopy.,Yajima H, Ogura T, Nitta R, Okada Y, Sato C, Hirokawa N J Cell Biol. 2012 Aug 6;198(3):315-22. doi: 10.1083/jcb.201201161. Epub 2012 Jul , 30. PMID:22851320<ref>PMID:22851320</ref>
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
==See Also==
-
</div>
+
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
-
</StructureSection>
+
</SX>
 +
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
-
[[Category: Hirokawa, N]]
+
[[Category: Hirokawa N]]
-
[[Category: Nitta, R]]
+
[[Category: Nitta R]]
-
[[Category: Ogura, T]]
+
[[Category: Ogura T]]
-
[[Category: Okada, Y]]
+
[[Category: Okada Y]]
-
[[Category: Sato, C]]
+
[[Category: Sato C]]
-
[[Category: Yajima, H]]
+
[[Category: Yajima H]]
-
[[Category: Gmpcpp]]
+
-
[[Category: Gtp-state structure]]
+
-
[[Category: Micotubule polymerization]]
+
-
[[Category: Microtubule]]
+
-
[[Category: Microtubule stabilaization]]
+
-
[[Category: Structural protein]]
+
-
[[Category: Tubulin]]
+

Current revision

Structure of alpha- and beta- tubulin in GMPCPP-microtubules

3j7i, resolution 8.90Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j7i"
Personal tools