This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4j40

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:01, 1 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa==
==Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa==
-
<StructureSection load='4j40' size='340' side='right' caption='[[4j40]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
+
<StructureSection load='4j40' size='340' side='right'caption='[[4j40]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4j40]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3hvb 3hvb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J40 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J40 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4j40]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3hvb 3hvb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J40 FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hv8|3hv8]], [[3hv9|3hv9]], [[3hva|3hva]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.99&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fimX, PA4959 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j40 OCA], [https://pdbe.org/4j40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j40 RCSB], [https://www.ebi.ac.uk/pdbsum/4j40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j40 ProSAT]</span></td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic-guanylate-specific_phosphodiesterase Cyclic-guanylate-specific phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.52 3.1.4.52] </span></td></tr>
+
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j40 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j40 RCSB], [http://www.ebi.ac.uk/pdbsum/4j40 PDBsum]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q9HUK6_PSEAE Q9HUK6_PSEAE]
-
Bacterial pathogenesis involves social behavior including biofilm formation and swarming, processes that are regulated by the bacterially unique second messenger cyclic di-GMP (c-di-GMP). Diguanylate cyclases containing GGDEF and phosphodiesterases containing EAL domains have been identified as the enzymes controlling cellular c-di-GMP levels, yet less is known regarding signal transmission and the targets of c-di-GMP. FimX, a protein from Pseudomonas aeruginosa that governs twitching motility, belongs to a large subfamily containing both GGDEF and EAL domains. Biochemical and structural analyses reveals its function as a high-affinity receptor for c-di-GMP. A model for full-length FimX was generated combining solution scattering data and crystal structures of the degenerate GGDEF and EAL domains. Although FimX forms a dimer in solution via the N-terminal domains, a crystallographic EAL domain dimer suggests modes for the regulation of FimX by c-di-GMP binding. The results provide the structural basis for c-di-GMP sensing via degenerate phosphodiesterases.
+
-
 
+
-
Structural analysis of the GGDEF-EAL domain-containing c-di-GMP receptor FimX.,Navarro MV, De N, Bae N, Wang Q, Sondermann H Structure. 2009 Aug 12;17(8):1104-16. PMID:19679088<ref>PMID:19679088</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Cyclic-guanylate-specific phosphodiesterase]]
+
[[Category: Large Structures]]
-
[[Category: Pseudomonas aeruginosa pao1]]
+
[[Category: Pseudomonas aeruginosa PAO1]]
-
[[Category: Bae, N]]
+
[[Category: Bae N]]
-
[[Category: De, N]]
+
[[Category: De N]]
-
[[Category: Navarro, M V]]
+
[[Category: Navarro MV]]
-
[[Category: Sondermann, H]]
+
[[Category: Sondermann H]]
-
[[Category: Wang, Q]]
+
[[Category: Wang Q]]
-
[[Category: Biofilm]]
+
-
[[Category: C-di-gmp]]
+
-
[[Category: C-di-gmp effector]]
+
-
[[Category: Eal phosphodiesterase]]
+
-
[[Category: Hydrolase]]
+

Current revision

Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa

PDB ID 4j40

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4j40"
Personal tools