This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1otf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:02, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1otf.jpg|left|200px]]
 
-
{{Structure
+
==4-OXALOCROTONATE TAUTOMERASE-TRICLINIC CRYSTAL FORM==
-
|PDB= 1otf |SIZE=350|CAPTION= <scene name='initialview01'>1otf</scene>, resolution 1.9&Aring;
+
<StructureSection load='1otf' size='340' side='right'caption='[[1otf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1otf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._CF600 Pseudomonas sp. CF600]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OTF FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
|GENE= DMPL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.])
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1otf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otf OCA], [https://pdbe.org/1otf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1otf RCSB], [https://www.ebi.ac.uk/pdbsum/1otf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1otf ProSAT]</span></td></tr>
-
}}
+
</table>
-
 
+
== Function ==
-
'''4-OXALOCROTONATE TAUTOMERASE-TRICLINIC CRYSTAL FORM'''
+
[https://www.uniprot.org/uniprot/4OT_PSEUF 4OT_PSEUF] Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
5-Carboxymethyl-2-hydroxymuconate isomerase (CHMI) and 4-oxalocrotonate tautomerase (4-OT) are enzymes that catalyze the isomerization of unsaturated ketones. They share a common enzyme mechanism, although they show a preference for different substrates. There is no apparent sequence homology between the enzymes. To investigate the molecular mechanism and the basis for their substrate specificity, we have determined the crystal structures of the two enzymes at high resolution. 4-OT is hexameric, with the subunits arranged with 32 symmetry. CHMI is trimeric and has extensive contacts between subunits, which include secondary structural elements. The central core of the CHMI monomer has a fold similar to a 4-OT dimer, but the secondary structural elements that form the subunit contacts around the 3-fold axis are different in the two enzymes. The region of greatest similarity between the two enzymes is a large pocket that is proposed to be the active site. The enzymes appear to operate via a "one-base" mechanism, and the possible role of residues in this pocket is discussed in view of this idea. Finally, the molecular basis for substrate specificity in the two enzymes is discussed.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1otf_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
1OTF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTF OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1otf ConSurf].
-
Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases., Subramanya HS, Roper DI, Dauter Z, Dodson EJ, Davies GJ, Wilson KS, Wigley DB, Biochemistry. 1996 Jan 23;35(3):792-802. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8547259 8547259]
+
<div style="clear:both"></div>
-
[[Category: Pseudomonas sp.]]
+
__TOC__
-
[[Category: Single protein]]
+
</StructureSection>
-
[[Category: Dauter, Z.]]
+
[[Category: Large Structures]]
-
[[Category: Davies, G J.]]
+
[[Category: Pseudomonas sp. CF600]]
-
[[Category: Dodson, E J.]]
+
[[Category: Dauter Z]]
-
[[Category: Roper, D I.]]
+
[[Category: Davies GJ]]
-
[[Category: Subramanya, H S.]]
+
[[Category: Dodson EJ]]
-
[[Category: Wigley, D B.]]
+
[[Category: Roper DI]]
-
[[Category: Wilson, K S.]]
+
[[Category: Subramanya HS]]
-
[[Category: tautomerase]]
+
[[Category: Wigley DB]]
-
 
+
[[Category: Wilson KS]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:15:23 2008''
+

Current revision

4-OXALOCROTONATE TAUTOMERASE-TRICLINIC CRYSTAL FORM

PDB ID 1otf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1otf"
Personal tools