Pseudouridine synthase

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{{STRUCTURE_1ze2| PDB=1ze2 | SIZE=400| SCENE= |right|CAPTION=tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate [[1ze2]] }}
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<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
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__TOC__
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==Function==
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[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:
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'''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine. PUS are active in the ribosomal large subunit (Rlu) and ribosomal small subunit (Rsu). '''Probable tRNA pseudouridine synthase''' (PPUS) is a similar enzyme.
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* [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.
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== 3D Structures of tRNA pseudouridine synthase==
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* [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization.
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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== Structural highlights ==
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{{#tree:id=OrganizedByTopic|openlevels=0|
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The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
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== 3D Structures of pseudouridine synthase ==
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[[Pseudouridine synthase 3D structures]]
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*tRNA pseudouridine synthase
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</StructureSection>
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**[[1dj0]] – EcPUS I – ''Escherichia coli''<BR />
 
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**[[1ksk]], [[1ksl]] – EcPUS RsuA<BR />
 
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**[[1vio]] - PUS RsuA – ''Haemophilus influenzae''<BR />
 
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**[[1xpi]] - EcPUS RluC catalytic domain<BR />
 
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**[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <BR />
 
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**[[2ist]] - EcPUS RluD <BR />
 
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**[[1prz]], [[1qyu]], [[1v9f]] - EcPUS RluD catalytic domain<BR />
 
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**[[2olw]] - EcPUS RluE<BR />
 
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**[[2oml]] - EcPUS RluE catalytic domain<BR />
 
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**[[2gml]] - EcPUS RluF catalytic domain<BR />
 
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**[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human<br />
 
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**[[4nz7]] - hPUS Pus1 catalytic domain (mutant)<br />
 
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**[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR />
 
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**[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR />
 
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**[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD
 
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*tRNA pseudouridine synthase complex with RNA
 
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**[[1k8w]] – EcPUS B + stem-loop RNA<BR />
 
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**[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<BR />
 
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**[[2i82]] – EcPUS RluA + RNA<BR />
 
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**[[3dh3]] - EcPUS RluF + RNA<BR />
 
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**[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<BR />
 
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**[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<BR />
 
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**[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
 
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**[[3hjy]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA<BR />
 
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**[[1ze2]] - TmPUS B + RNA<BR />
 
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**[[2ab4]] - TmPUS B (mutant) + RNA<BR />
 
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**[[1r3e]], [[1r3f]] - EcPUS B + RNA<BR />
 
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*Probable tRNA pseudouridine synthase
 
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**[[2apo]] – PPUS + ribosome biogenesis protein Nop10 – ''Methanocaldococcus jannaschii''<br />
 
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**[[2ey4]] - PfPPUS Cbf5 + ribosome biogenesis protein Nop10 + small nucleolar RNP <br />
 
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**[[2hvy]], [[3hax]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA<br />
 
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**[[2rfk]] - PfPUS B + ribosome biogenesis protein Nop10 + small nucleolar RNP + RNA<br />
 
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**[[3hay]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + small nucleolar RNP + RNA<br />
 
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**[[2aus]] – PPUS B + ribosome biogenesis protein Nop10 – ''Pyrococcus abyssi''<br />
 
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**[[1z2z]] – PPUS D – ''Methanosarcina mazei''<br />
 
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}}
 
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[[Category:Topic Page]]

Current revision

tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate 1ze2

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