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1ou9

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[[Image:1ou9.jpg|left|200px]]
 
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{{Structure
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==Structure of SspB, a AAA+ protease delivery protein==
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|PDB= 1ou9 |SIZE=350|CAPTION= <scene name='initialview01'>1ou9</scene>, resolution 1.80&Aring;
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<StructureSection load='1ou9' size='340' side='right'caption='[[1ou9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1ou9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OU9 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE= SSPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ou9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ou9 OCA], [https://pdbe.org/1ou9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ou9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ou9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ou9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSPB_HAEIN SSPB_HAEIN] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth (By similarity). Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ou/1ou9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ou9 ConSurf].
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<div style="clear:both"></div>
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'''Structure of SspB, a AAA+ protease delivery protein'''
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==See Also==
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*[[Stringent starvation protein 3D structures|Stringent starvation protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.
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==About this Structure==
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1OU9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OU9 OCA].
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==Reference==
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Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag., Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA, Mol Cell. 2003 Aug;12(2):365-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14536076 14536076]
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[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Baker, T A.]]
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[[Category: Baker TA]]
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[[Category: Grant, R A.]]
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[[Category: Grant RA]]
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[[Category: Levchenko, I.]]
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[[Category: Levchenko I]]
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[[Category: Sauer, R T.]]
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[[Category: Sauer RT]]
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[[Category: Wah, D A.]]
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[[Category: Wah DA]]
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[[Category: CA]]
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[[Category: homodimer]]
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[[Category: ssra peptide-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:15:41 2008''
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Current revision

Structure of SspB, a AAA+ protease delivery protein

PDB ID 1ou9

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