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4r1d

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==The crystal structure of Tle4-Tli4 complex==
==The crystal structure of Tle4-Tli4 complex==
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<StructureSection load='4r1d' size='340' side='right' caption='[[4r1d]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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<StructureSection load='4r1d' size='340' side='right'caption='[[4r1d]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4r1d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R1D FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4r1d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R1D FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.749&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r1d RCSB], [http://www.ebi.ac.uk/pdbsum/4r1d PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1d OCA], [https://pdbe.org/4r1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r1d RCSB], [https://www.ebi.ac.uk/pdbsum/4r1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1d ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9I3K2_PSEAE Q9I3K2_PSEAE]
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The type VI secretion system (T6SS) has recently been demonstrated to mediate interbacterial competition and to discriminate between self and nonself. T6SS(+) bacteria employ toxic effectors to inhibit rival cells and concurrently use effector cognate immunity proteins to protect their sibling cells. The effector and immunity pairs (E-I pairs) endow the bacteria with a great advantage in niche competition. Tle4-Tli4 (PA1510-PA1509) is a newly identified E-I pair that is controlled by H2-T6SS in Pseudomonas aeruginosa. Tle4 exhibits phospholipase activity, which destroys the cell membrane of rival cells, and the periplasm-located Tli4 in donor cells eliminates this toxic effect of Tle4. In this paper, the structure of the Tle4-Tli4 complex is reported at 1.75 A resolution. Tle4 consists of two domains: a conserved alpha/beta-hydrolase domain and an unusual cap domain in which two lid regions (lid1 and lid2) display a closed conformation that buries the catalytic triad in a deep funnel. Tli4 also displays a two-domain structure, in which a large lobe and a small lobe form a crab claw-like conformation. Tli4 uses this crab claw to grasp the cap domain of Tle4, especially the lid2 region, which prevents the interfacial activation of Tle4 and thus causes enzymatic dysfunction of Tle4 in sister cells.
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The structural basis of the Tle4-Tli4 complex reveals the self-protection mechanism of H2-T6SS in Pseudomonas aeruginosa.,Lu D, Zheng Y, Liao N, Wei L, Xu B, Liu X, Liu J Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3233-43. doi:, 10.1107/S1399004714023967. Epub 2014 Nov 22. PMID:25478841<ref>PMID:25478841</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lu, D]]
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[[Category: Large Structures]]
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[[Category: Hydrolase-hydrolase inhibitor complex]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Lipase]]
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[[Category: Lu D]]

Current revision

The crystal structure of Tle4-Tli4 complex

PDB ID 4r1d

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