4wl9

From Proteopedia

(Difference between revisions)

Current revision

Time Resolved Serial Femtosecond Crystallography Captures High Resolution Intermediates of PYP

Structural highlights

4wl9 is a 1 chain structure with sequence from Halorhodospira halophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYP_HALHA Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.

Publication Abstract from PubMed

Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.,Tenboer J, Basu S, Zatsepin N, Pande K, Milathianaki D, Frank M, Hunter M, Boutet S, Williams GJ, Koglin JE, Oberthuer D, Heymann M, Kupitz C, Conrad C, Coe J, Roy-Chowdhury S, Weierstall U, James D, Wang D, Grant T, Barty A, Yefanov O, Scales J, Gati C, Seuring C, Srajer V, Henning R, Schwander P, Fromme R, Ourmazd A, Moffat K, Van Thor JJ, Spence JC, Fromme P, Chapman HN, Schmidt M Science. 2014 Dec 5;346(6214):1242-6. doi: 10.1126/science.1259357. PMID:25477465^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tenboer J, Basu S, Zatsepin N, Pande K, Milathianaki D, Frank M, Hunter M, Boutet S, Williams GJ, Koglin JE, Oberthuer D, Heymann M, Kupitz C, Conrad C, Coe J, Roy-Chowdhury S, Weierstall U, James D, Wang D, Grant T, Barty A, Yefanov O, Scales J, Gati C, Seuring C, Srajer V, Henning R, Schwander P, Fromme R, Ourmazd A, Moffat K, Van Thor JJ, Spence JC, Fromme P, Chapman HN, Schmidt M. Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. Science. 2014 Dec 5;346(6214):1242-6. doi: 10.1126/science.1259357. PMID:25477465 doi:http://dx.doi.org/10.1126/science.1259357

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wl9"

Categories: Halorhodospira halophila | Large Structures | Schmidt M | Tenboer J

@@ Line 1: / Line 1: @@
 ==Time Resolved Serial Femtosecond Crystallography Captures High Resolution Intermediates of PYP==
-<StructureSection load='4wl9' size='340' side='right' caption='[[4wl9]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+<StructureSection load='4wl9' size='340' side='right'caption='[[4wl9]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4wl9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WL9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4wl9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WL9 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wla|4wla]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wl9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wl9 RCSB], [http://www.ebi.ac.uk/pdbsum/4wl9 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wl9 OCA], [https://pdbe.org/4wl9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wl9 RCSB], [https://www.ebi.ac.uk/pdbsum/4wl9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wl9 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4wl9" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Schmidt, M]]
+[[Category: Halorhodospira halophila]]
-[[Category: Tenboer, J]]
+[[Category: Large Structures]]
-[[Category: Blue-light photoreceptor]]
+[[Category: Schmidt M]]
-[[Category: Dark structure]]
+[[Category: Tenboer J]]
-[[Category: Microcrystal]]
-[[Category: Nanocrystal]]
-[[Category: Time-resolved serial femtosecond crystallography]]

4wl9

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Current revision

Time Resolved Serial Femtosecond Crystallography Captures High Resolution Intermediates of PYP

Structural highlights

Function

Publication Abstract from PubMed

References

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