4qmk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (17:33, 20 September 2023) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of type III effector protein ExoU (exoU)==
==Crystal structure of type III effector protein ExoU (exoU)==
-
<StructureSection load='4qmk' size='340' side='right' caption='[[4qmk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+
<StructureSection load='4qmk' size='340' side='right'caption='[[4qmk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4qmk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QMK FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4qmk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens_A506 Pseudomonas fluorescens A506]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QMK FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qmk RCSB], [http://www.ebi.ac.uk/pdbsum/4qmk PDBsum]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmk OCA], [https://pdbe.org/4qmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qmk RCSB], [https://www.ebi.ac.uk/pdbsum/4qmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmk ProSAT]</span></td></tr>
</table>
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Bacterial toxins require localization to specific intracellular compartments following injection into host cells. In this study, we examine the membrane targeting of a broad family of bacterial proteins, the patatin-like phospholipases. The best-characterized member of this family is ExoU, an effector of the Pseudomonas aeruginosa type III secretion system. Upon injection into host cells, ExoU localizes to the plasma membrane, where it uses its phospholipase A2 activity to lyse infected cells. The targeting mechanism of ExoU is poorly characterized, but it was recently found to bind to the phospholipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a marker for the plasma membrane of eukaryotic cells. We confirmed that the membrane localization domain (MLD) of ExoU had direct affinity for PI(4,5)P2 and determined that this binding was required for ExoU localization. Previously uncharacterized ExoU homologs from Pseudomonas fluorescens and Photorhabdus asymbiotica also localized to the plasma membrane and required PI(4,5)P2 for this localization. A conserved arginine within the MLD was critical for interaction of each protein with PI(4,5)P2 and for localization. Further, we determined the crystal structure of the full-length P. fluorescens ExoU and found that it was similar to that of P. aeruginosa ExoU. Each MLD contains a four-helical bundle, with the conserved arginine exposed at its cap to allow for interaction with the negatively charged PI(4,5)P2. Overall, these findings provide a structural explanation for the targeting of patatin-like phospholipases to the plasma membrane and define the MLD of ExoU as a member of a new class of PI(4,5)P2 binding domains.
 +
 +
A Novel Phosphatidylinositol 4,5-bisphosphate Binding Domain Mediates Plasma Membrane Localization of ExoU and Other Patatin-like Phospholipases.,Tyson GH, Halavaty AS, Kim H, Geissler B, Agard M, Satchell KJ, Cho W, Anderson WF, Hauser AR J Biol Chem. 2014 Dec 10. pii: jbc.M114.611251. PMID:25505182<ref>PMID:25505182</ref>
 +
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 4qmk" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Anderson, W F]]
+
[[Category: Large Structures]]
-
[[Category: Structural genomic]]
+
[[Category: Pseudomonas fluorescens A506]]
-
[[Category: Halavaty, A S]]
+
[[Category: Anderson WF]]
-
[[Category: Hauser, A R]]
+
[[Category: Halavaty AS]]
-
[[Category: Tyson, G H]]
+
[[Category: Hauser AR]]
-
[[Category: Zhang, A]]
+
[[Category: Tyson GH]]
-
[[Category: Csgid]]
+
[[Category: Zhang A]]
-
[[Category: Infectious disease]]
+
-
[[Category: Membrane localization domain]]
+
-
[[Category: National institute of allergy and infectious disease]]
+
-
[[Category: Niaid]]
+
-
[[Category: Patatin-like phospholipase domain]]
+
-
[[Category: Pla2 domain]]
+
-
[[Category: Pseudomonas fluorescens a506]]
+
-
[[Category: Toxin]]
+
-
[[Category: Type iii effector protein exou]]
+
-
[[Category: Type iii secretion system]]
+

Current revision

Crystal structure of type III effector protein ExoU (exoU)

PDB ID 4qmk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools