1p49

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[[Image:1p49.jpg|left|200px]]
 
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{{Structure
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==Structure of Human Placental Estrone/DHEA Sulfatase==
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|PDB= 1p49 |SIZE=350|CAPTION= <scene name='initialview01'>1p49</scene>, resolution 2.60&Aring;
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<StructureSection load='1p49' size='340' side='right'caption='[[1p49]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1p49]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P49 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Steryl-sulfatase Steryl-sulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.2 3.1.6.2]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALS:(3S)-3-(SULFOOXY)-L-SERINE'>ALS</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p49 OCA], [https://pdbe.org/1p49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p49 RCSB], [https://www.ebi.ac.uk/pdbsum/1p49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p49 ProSAT]</span></td></tr>
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</table>
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'''Structure of Human Placental Estrone/DHEA Sulfatase'''
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== Disease ==
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[https://www.uniprot.org/uniprot/STS_HUMAN STS_HUMAN] Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:[https://omim.org/entry/308100 308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks.<ref>PMID:1539590</ref> <ref>PMID:9252398</ref> <ref>PMID:10679952</ref> <ref>PMID:10844566</ref>
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== Function ==
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==Overview==
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[https://www.uniprot.org/uniprot/STS_HUMAN STS_HUMAN] Conversion of sulfated steroid precursors to estrogens during pregnancy.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/1p49_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p49 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Estrone sulfatase (ES; 562 amino acids), one of the key enzymes responsible for maintaining high levels of estrogens in breast tumor cells, is associated with the membrane of the endoplasmic reticulum (ER). The structure of ES, purified from the microsomal fraction of human placentas, has been determined at 2.60-A resolution by x-ray crystallography. This structure shows a domain consisting of two antiparallel alpha-helices that protrude from the roughly spherical molecule, thereby giving the molecule a "mushroom-like" shape. These highly hydrophobic helices, each about 40 A long, are capable of traversing the membrane, thus presumably anchoring the functional domain on the membrane surface facing the ER lumen. The location of the transmembrane domain is such that the opening to the active site, buried deep in a cavity of the "gill" of the "mushroom," rests near the membrane surface, thereby suggesting a role of the lipid bilayer in catalysis. This simple architecture could be a prototype utilized by the ER membrane in dictating the form and the function of ER-resident enzymes.
Estrone sulfatase (ES; 562 amino acids), one of the key enzymes responsible for maintaining high levels of estrogens in breast tumor cells, is associated with the membrane of the endoplasmic reticulum (ER). The structure of ES, purified from the microsomal fraction of human placentas, has been determined at 2.60-A resolution by x-ray crystallography. This structure shows a domain consisting of two antiparallel alpha-helices that protrude from the roughly spherical molecule, thereby giving the molecule a "mushroom-like" shape. These highly hydrophobic helices, each about 40 A long, are capable of traversing the membrane, thus presumably anchoring the functional domain on the membrane surface facing the ER lumen. The location of the transmembrane domain is such that the opening to the active site, buried deep in a cavity of the "gill" of the "mushroom," rests near the membrane surface, thereby suggesting a role of the lipid bilayer in catalysis. This simple architecture could be a prototype utilized by the ER membrane in dictating the form and the function of ER-resident enzymes.
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==Disease==
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Structure of human estrone sulfatase suggests functional roles of membrane association.,Hernandez-Guzman FG, Higashiyama T, Pangborn W, Osawa Y, Ghosh D J Biol Chem. 2003 Jun 20;278(25):22989-97. Epub 2003 Mar 25. PMID:12657638<ref>PMID:12657638</ref>
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Known diseases associated with this structure: Ichthyosis, X-linked OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=308100 308100]], Placental steroid sulfatase deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=308100 308100]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1P49 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P49 OCA].
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</div>
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<div class="pdbe-citations 1p49" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structure of human estrone sulfatase suggests functional roles of membrane association., Hernandez-Guzman FG, Higashiyama T, Pangborn W, Osawa Y, Ghosh D, J Biol Chem. 2003 Jun 20;278(25):22989-97. Epub 2003 Mar 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12657638 12657638]
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*[[Sulfatase 3D structures|Sulfatase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Steryl-sulfatase]]
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[[Category: Ghosh D]]
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[[Category: Ghosh, D.]]
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[[Category: Hernandez-Guzman FG]]
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[[Category: Hernandez-Guzman, F G.]]
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[[Category: Higashiyama T]]
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[[Category: Higashiyama, T.]]
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[[Category: Osawa Y]]
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[[Category: Osawa, Y.]]
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[[Category: Pangborn W]]
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[[Category: Pangborn, W.]]
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[[Category: BOG]]
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[[Category: CA]]
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[[Category: NAG]]
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[[Category: PO4]]
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[[Category: dehydroepiandrosterone sulfate]]
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[[Category: endoplasmic reticulum membrane-bound]]
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[[Category: estrone sulfate]]
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[[Category: human placental enzyme]]
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[[Category: steroid biosynthesis]]
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[[Category: steroid sulfatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:19:39 2008''
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Current revision

Structure of Human Placental Estrone/DHEA Sulfatase

PDB ID 1p49

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