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1p5v

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[[Image:1p5v.jpg|left|200px]]
 
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{{Structure
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==X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex==
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|PDB= 1p5v |SIZE=350|CAPTION= <scene name='initialview01'>1p5v</scene>, resolution 1.70&Aring;
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<StructureSection load='1p5v' size='340' side='right'caption='[[1p5v]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1p5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P5V FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= CAF1M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis]), CAF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p5v OCA], [https://pdbe.org/1p5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p5v RCSB], [https://www.ebi.ac.uk/pdbsum/1p5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p5v ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex'''
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[https://www.uniprot.org/uniprot/CAF1M_YERPE CAF1M_YERPE] Has a stimulatory role for the envelope antigen F1 secretion. It seems to interact with the subunit polypeptide and to prevent it from digestion by a protease.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding to snapshots of the assembly process reveal the structural basis of fiber formation. Comparison of chaperone bound Caf1 subunit with the subunit in the fiber reveals a novel type of conformational change involving the entire hydrophobic core of the protein. The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p5/1p5v_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1P5V is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5V OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p5v ConSurf].
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Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12787500 12787500]
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<div style="clear:both"></div>
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[[Category: Protein complex]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Yersinia pestis]]
[[Category: Yersinia pestis]]
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[[Category: Berglund, J.]]
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[[Category: Berglund J]]
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[[Category: Fooks, L J.]]
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[[Category: Fooks LJ]]
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[[Category: Ibrahim, T M.]]
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[[Category: Ibrahim TM]]
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[[Category: Knight, S D.]]
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[[Category: Knight SD]]
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[[Category: MacIntyre, S.]]
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[[Category: MacIntyre S]]
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[[Category: Pudney, A F.]]
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[[Category: Pudney AF]]
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[[Category: Zavialov, A V.]]
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[[Category: Zavialov AV]]
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[[Category: chaperone]]
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[[Category: chaperone-subunit complex]]
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[[Category: chaperone-target complex]]
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[[Category: donor strand complementation]]
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[[Category: donor strand exchange]]
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[[Category: protein fiber]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:20:13 2008''
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Current revision

X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex

PDB ID 1p5v

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