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| | ==NMR structure of chicken AvBD2-K31A mutant== | | ==NMR structure of chicken AvBD2-K31A mutant== |
| - | <StructureSection load='2lg6' size='340' side='right' caption='[[2lg6]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2lg6' size='340' side='right'caption='[[2lg6]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lg6]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LG6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lg6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LG6 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lg5|2lg5]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lg6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lg6 RCSB], [http://www.ebi.ac.uk/pdbsum/2lg6 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lg6 OCA], [https://pdbe.org/2lg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lg6 RCSB], [https://www.ebi.ac.uk/pdbsum/2lg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lg6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GLL2_CHICK GLL2_CHICK] Potent antibacterial activity against the Gram-negative bacterium E.coli ML-35, and against the Gram-positive bacterium L.monocytogenes EGD. Lacks antifungal activity against C.albicans.<ref>PMID:8150085</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 2lg6" style="background-color:#fffaf0;"></div> |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Defensin|Defensin]] | + | *[[Defensin 3D structures|Defensin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Landon, C]] | + | [[Category: Gallus gallus]] |
| - | [[Category: Meudal, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Antimicrobial protein]] | + | [[Category: Landon C]] |
| | + | [[Category: Meudal H]] |
| Structural highlights
Function
GLL2_CHICK Potent antibacterial activity against the Gram-negative bacterium E.coli ML-35, and against the Gram-positive bacterium L.monocytogenes EGD. Lacks antifungal activity against C.albicans.[1]
Publication Abstract from PubMed
Numerous beta-defensins have been identified in birds, and the potential use of these peptides as alternatives to antibiotics has been proposed, in particular to fight antibiotic-resistant and zoonotic bacterial species. Little is known about the mechanism of antibacterial activity of avian beta-defensins, and this study was carried out to obtain initial insights into the involvement of structural features or specific residues in the antimicrobial activity of chicken AvBD2. Chicken AvBD2 and its enantiomeric counterpart were chemically synthesized. Peptide elongation and oxidative folding were both optimized. The similar antimicrobial activity measured for both L- and D-proteins clearly indicates that there is no chiral partner. Therefore, the bacterial membrane is in all likelihood the primary target. Moreover, this work indicates that the three-dimensional fold is required for an optimal antimicrobial activity, in particular for gram-positive bacterial strains. The three-dimensional NMR structure of chicken AvBD2 defensin displays the structural three-stranded antiparallel beta-sheet characteristic of beta-defensins. The surface of the molecule does not display any amphipathic character. In light of this new structure and of the king penguin AvBD103b defensin structure, the consensus sequence of the avian beta-defensin family was analyzed. Well conserved residues were highlighted, and the potential strategic role of the lysine 31 residue of AvBD2 was emphasized. The synthetic AvBD2-K31A variant displayed substantial N-terminal structural modifications and a dramatic decrease in activity. Taken together, these results demonstrate the structural as well as the functional role of the critical lysine 31 residue in antimicrobial activity.
Initial insights into structure-activity relationships of avian defensins.,Derache C, Meudal H, Aucagne V, Mark KJ, Cadene M, Delmas AF, Lalmanach AC, Landon C J Biol Chem. 2012 Mar 2;287(10):7746-55. Epub 2011 Dec 27. PMID:22205704[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Harwig SS, Swiderek KM, Kokryakov VN, Tan L, Lee TD, Panyutich EA, Aleshina GM, Shamova OV, Lehrer RI. Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes. FEBS Lett. 1994 Apr 11;342(3):281-5. PMID:8150085
- ↑ Derache C, Meudal H, Aucagne V, Mark KJ, Cadene M, Delmas AF, Lalmanach AC, Landon C. Initial insights into structure-activity relationships of avian defensins. J Biol Chem. 2012 Mar 2;287(10):7746-55. Epub 2011 Dec 27. PMID:22205704 doi:10.1074/jbc.M111.312108
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