1p9o

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[[Image:1p9o.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Phosphopantothenoylcysteine Synthetase==
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|PDB= 1p9o |SIZE=350|CAPTION= <scene name='initialview01'>1p9o</scene>, resolution 2.30&Aring;
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<StructureSection load='1p9o' size='340' side='right'caption='[[1p9o]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1p9o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9O FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphopantothenate--cysteine_ligase Phosphopantothenate--cysteine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.5 6.3.2.5]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= coaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9o OCA], [https://pdbe.org/1p9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9o RCSB], [https://www.ebi.ac.uk/pdbsum/1p9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9o ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of Phosphopantothenoylcysteine Synthetase'''
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== Function ==
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[https://www.uniprot.org/uniprot/PPCS_HUMAN PPCS_HUMAN] Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.<ref>PMID:11923312</ref> <ref>PMID:12906824</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The structure of human phosphopantothenoylcysteine (PPC) synthetase was determined at 2.3 A resolution. PPC synthetase is a dimer with identical monomers. Some features of the monomer fold resemble a group of NAD-dependent enzymes, while other features resemble the ribokinase fold. The ATP, phosphopantothenate, and cysteine binding sites were deduced from modeling studies. Highly conserved ATP binding residues include Gly43, Ser61, Gly63, Gly66, Phe230, and Asn258. Highly conserved phosphopantothenate binding residues include Asn59, Ala179, Ala180, and Asp183 from one monomer and Arg55' from the adjacent monomer. The structure predicts a ping pong mechanism with initial formation of an acyladenylate intermediate, followed by release of pyrophosphate and attack by cysteine to form the final products PPC and AMP.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9o_consurf.spt"</scriptWhenChecked>
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1P9O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9O OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution., Manoj N, Strauss E, Begley TP, Ealick SE, Structure. 2003 Aug;11(8):927-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12906824 12906824]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9o ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphopantothenate--cysteine ligase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Begley TP]]
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[[Category: Begley, T P.]]
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[[Category: Ealick SE]]
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[[Category: Ealick, S E.]]
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[[Category: Manoj N]]
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[[Category: Manoj, N.]]
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[[Category: Strauss E]]
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[[Category: Strauss, E.]]
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[[Category: SO4]]
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[[Category: synthetase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:21:42 2008''
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Current revision

Crystal Structure of Phosphopantothenoylcysteine Synthetase

PDB ID 1p9o

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