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1pam

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[[Image:1pam.gif|left|200px]]
 
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{{Structure
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==CYCLODEXTRIN GLUCANOTRANSFERASE==
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|PDB= 1pam |SIZE=350|CAPTION= <scene name='initialview01'>1pam</scene>, resolution 1.80&Aring;
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<StructureSection load='1pam' size='340' side='right'caption='[[1pam]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1pam]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._1011 Bacillus sp. 1011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PAM FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pam OCA], [https://pdbe.org/1pam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pam RCSB], [https://www.ebi.ac.uk/pdbsum/1pam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pam ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDGT_BACS0 CDGT_BACS0]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pa/1pam_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pam ConSurf].
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<div style="clear:both"></div>
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'''CYCLODEXTRIN GLUCANOTRANSFERASE'''
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==See Also==
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Cyclodextrin glucanotransferase (CGTase) is an enzyme which produces cyclodextrins by the degradation of starch. The enzyme from alkalophilic Bacillus sp. 1011, consisting of 686 amino acid residues, was crystallized from the solution containing 20% PEG 3000 and 20% 2-propanol at pH 5.6 adjusted with citrate buffer. The space group was P1 and the unit cell contained two molecules (V(m) = 2.41 A(3) Da(-1)). The structure was solved by the molecular replacement method and refined to a conventional R value of 0.161 (R(free) = 0.211) for the reflections in the resolution range 1.8-10 A by energy minimization combined with simulated annealing. The molecule consists of five domains, designated A-E, and its backbone structure is similar to the structure of other bacterial CGTases. The molecule has two calcium binding sites where calcium ions are coordinated by seven ligands, forming a distorted pentagonal bipyramid. The two independent molecules are related by a pseudotwofold symmetry and are superimposed with an r.m.s. deviation value of 0.32 A for equivalent C(alpha) atoms. Comparison of these molecules indicated the relatively large mobility of domains C and E with respect to domain A. The active site is filled with water molecules forming a hydrogen-bond network with polar side-chain groups. Two water molecules commonly found in the active center of both molecules link to several catalytically important residues by hydrogen bonds and participate in maintaining a similar orientation of side chains in the two independent molecules.
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[[Category: Bacillus sp. 1011]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Aoyagi M]]
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1PAM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAM OCA].
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[[Category: Haga K]]
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[[Category: Harata K]]
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==Reference==
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[[Category: Nakamura A]]
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X-ray structure of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011. Comparison of two independent molecules at 1.8 A resolution., Harata K, Haga K, Nakamura A, Aoyagi M, Yamane K, Acta Crystallogr D Biol Crystallogr. 1996 Nov 1;52(Pt 6):1136-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299574 15299574]
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[[Category: Yamane K]]
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[[Category: Bacillus sp.]]
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[[Category: Cyclomaltodextrin glucanotransferase]]
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[[Category: Single protein]]
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[[Category: Aoyagi, M.]]
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[[Category: Haga, K.]]
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[[Category: Harata, K.]]
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[[Category: Nakamura, A.]]
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[[Category: Yamane, K.]]
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[[Category: CA]]
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[[Category: calcium]]
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[[Category: glycosyltransferase]]
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[[Category: signal]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:21:59 2008''
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CYCLODEXTRIN GLUCANOTRANSFERASE

PDB ID 1pam

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