1pmy

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[[Image:1pmy.gif|left|200px]]
 
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{{Structure
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==REFINED CRYSTAL STRUCTURE OF PSEUDOAZURIN FROM METHYLOBACTERIUM EXTORQUENS AM1 AT 1.5 ANGSTROMS RESOLUTION==
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|PDB= 1pmy |SIZE=350|CAPTION= <scene name='initialview01'>1pmy</scene>, resolution 1.5&Aring;
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<StructureSection load='1pmy' size='340' side='right'caption='[[1pmy]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene>
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<table><tr><td colspan='2'>[[1pmy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PMY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pmy OCA], [https://pdbe.org/1pmy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pmy RCSB], [https://www.ebi.ac.uk/pdbsum/1pmy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pmy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUP_METEA AZUP_METEA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pm/1pmy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pmy ConSurf].
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<div style="clear:both"></div>
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'''REFINED CRYSTAL STRUCTURE OF PSEUDOAZURIN FROM METHYLOBACTERIUM EXTORQUENS AM1 AT 1.5 ANGSTROMS RESOLUTION'''
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==See Also==
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*[[Pseudoazurin|Pseudoazurin]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of pseudoazurin from Methylobacterium extorquens AM1 (PAZAM1) has been solved by the molecular replacement method using copper-copper distances as translation parameters, which were obtained from difference Patterson maps calculated with the synchrotron radiation data containing the multiwavelength anomalous-dispersion effect. The structure refinement was carried out by the use of molecular dynamics optimization and the restrained least-squares method. The final crystallographic R factor was 19.9% for the 14 365 reflections greater than 3sigma between 1.5 and 8.0 A resolution. This report describes the characteristic features of the structure of PAZAM 1 as well as the effectiveness of synchrotron radiation for structure analysis of metalloproteins. The environment of the metal active site and the structural differences among blue-copper proteins are discussed.
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[[Category: Large Structures]]
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[[Category: Methylorubrum extorquens]]
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==About this Structure==
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[[Category: Harada S]]
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1PMY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMY OCA].
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[[Category: Inoue T]]
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[[Category: Kai Y]]
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==Reference==
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[[Category: Kasai N]]
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Refined crystal structure of pseudoazurin from Methylobacterium extorquens AM1 at 1.5 A resolution., Inoue T, Kai Y, Harada S, Kasai N, Ohshiro Y, Suzuki S, Kohzuma T, Tobari J, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):317-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299445 15299445]
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[[Category: Kohzuma T]]
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[[Category: Methylobacterium extorquens]]
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[[Category: Ohshiro Y]]
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[[Category: Single protein]]
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[[Category: Suzuki S]]
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[[Category: Harada, S.]]
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[[Category: Tobari J]]
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[[Category: Inoue, T.]]
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[[Category: Kai, Y.]]
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[[Category: Kasai, N.]]
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[[Category: Kohzuma, T.]]
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[[Category: Ohshiro, Y.]]
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[[Category: Suzuki, S.]]
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[[Category: Tobari, J.]]
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[[Category: CU]]
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[[Category: electron transfer(cuproprotein)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:26:25 2008''
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Current revision

REFINED CRYSTAL STRUCTURE OF PSEUDOAZURIN FROM METHYLOBACTERIUM EXTORQUENS AM1 AT 1.5 ANGSTROMS RESOLUTION

PDB ID 1pmy

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