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1pre

From Proteopedia

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[[Image:1pre.gif|left|200px]]
 
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{{Structure
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==PROAEROLYSIN==
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|PDB= 1pre |SIZE=350|CAPTION= <scene name='initialview01'>1pre</scene>, resolution 2.8&Aring;
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<StructureSection load='1pre' size='340' side='right'caption='[[1pre]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1pre]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRE FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pre OCA], [https://pdbe.org/1pre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pre RCSB], [https://www.ebi.ac.uk/pdbsum/1pre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pre ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AERA_AERHY AERA_AERHY] Aerolysin is a cytolytic toxin exported by the Gram negative Aeromonas bacteria. The mature toxin binds to eukaryotic cells and aggregates to form holes approximately 3 nm in diameter, leading to destruction of the membrane permeability barrier and osmotic lysis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1pre_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pre ConSurf].
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<div style="clear:both"></div>
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'''PROAEROLYSIN'''
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==See Also==
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*[[Aerolysin|Aerolysin]]
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*[[Aerolysin 3D structures|Aerolysin 3D structures]]
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==Overview==
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__TOC__
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Aerolysin is chiefly responsible for the pathogenicity of Aeromonas hydrophila, a bacterium associated with diarrhoeal diseases and deep wound infections. Like many other microbial toxins, the protein changes in a multistep process from a completely water-soluble form to produce a transmembrane channel that destroys sensitive cells by breaking their permeability barriers. Here we describe the structure of proaerolysin determined by X-ray crystallography at 2.8 A resolution. The protoxin (M(r) 52,000) adopts a novel protein fold. Images of an aerolysin oligomer derived from electron microscopy have assisted in constructing a model of the membrane channel and have led to the proposal of a scheme to account for insertion of the protein into lipid bilayers to form ion channels.
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</StructureSection>
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==About this Structure==
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1PRE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRE OCA].
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==Reference==
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Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states., Parker MW, Buckley JT, Postma JP, Tucker AD, Leonard K, Pattus F, Tsernoglou D, Nature. 1994 Jan 20;367(6460):292-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7510043 7510043]
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[[Category: Aeromonas hydrophila]]
[[Category: Aeromonas hydrophila]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Buckley, J T.]]
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[[Category: Buckley JT]]
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[[Category: Parker, M W.]]
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[[Category: Parker MW]]
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[[Category: Postma, J P.M.]]
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[[Category: Postma JPM]]
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[[Category: Tsernoglou, D.]]
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[[Category: Tsernoglou D]]
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[[Category: Tucker, A D.]]
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[[Category: Tucker AD]]
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[[Category: signal]]
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[[Category: toxin (hemolytic polypeptide)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:28:03 2008''
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Current revision

PROAEROLYSIN

PDB ID 1pre

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