1pw4

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[[Image:1pw4.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of the Glycerol-3-Phosphate Transporter from E.Coli==
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|PDB= 1pw4 |SIZE=350|CAPTION= <scene name='initialview01'>1pw4</scene>, resolution 3.3&Aring;
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<StructureSection load='1pw4' size='340' side='right'caption='[[1pw4]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1pw4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PW4 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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|GENE= glpT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pw4 OCA], [https://pdbe.org/1pw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pw4 RCSB], [https://www.ebi.ac.uk/pdbsum/1pw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pw4 ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Crystal Structure of the Glycerol-3-Phosphate Transporter from E.Coli'''
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[https://www.uniprot.org/uniprot/GLPT_ECOLI GLPT_ECOLI] Responsible for glycerol-3-phosphate uptake.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The major facilitator superfamily represents the largest group of secondary membrane transporters in the cell. Here we report the 3.3 angstrom resolution structure of a member of this superfamily, GlpT, which transports glycerol-3-phosphate into the cytoplasm and inorganic phosphate into the periplasm. The amino- and carboxyl-terminal halves of the protein exhibit a pseudo two-fold symmetry. Closed off to the periplasm, a centrally located substrate-translocation pore contains two arginines at its closed end, which comprise the substrate-binding site. Upon substrate binding, the protein adopts a more compact conformation. We propose that GlpT operates by a single-binding site, alternating-access mechanism through a rocker-switch type of movement.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pw/1pw4_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1PW4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PW4 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pw4 ConSurf].
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Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli., Huang Y, Lemieux MJ, Song J, Auer M, Wang DN, Science. 2003 Aug 1;301(5633):616-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12893936 12893936]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Auer, M.]]
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[[Category: Auer M]]
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[[Category: Huang, Y.]]
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[[Category: Huang Y]]
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[[Category: Lemieux, M J.]]
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[[Category: Lemieux MJ]]
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[[Category: Song, J.]]
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[[Category: Song J]]
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[[Category: Wang, D N.]]
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[[Category: Wang DN]]
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[[Category: glycerol-3-phosphate]]
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[[Category: inner membrane]]
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[[Category: major facilitator superfamily]]
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[[Category: secondary active membrane transporter]]
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[[Category: transmembrane]]
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[[Category: transporter]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:29:46 2008''
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Current revision

Crystal Structure of the Glycerol-3-Phosphate Transporter from E.Coli

PDB ID 1pw4

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