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1pys

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[[Image:1pys.jpg|left|200px]]
 
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{{Structure
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==PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS==
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|PDB= 1pys |SIZE=350|CAPTION= <scene name='initialview01'>1pys</scene>, resolution 2.9&Aring;
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<StructureSection load='1pys' size='340' side='right'caption='[[1pys]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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<table><tr><td colspan='2'>[[1pys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PYS FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phenylalanine--tRNA_ligase Phenylalanine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.20 6.1.1.20]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pys OCA], [https://pdbe.org/1pys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pys RCSB], [https://www.ebi.ac.uk/pdbsum/1pys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pys ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYFA_THET8 SYFA_THET8]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/py/1pys_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pys ConSurf].
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<div style="clear:both"></div>
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'''PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS'''
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==See Also==
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of phenylalanyl-tRNA synthetase from Thermus thermophilus, solved at 2.9 A resolution, displays (alpha beta)2 subunit organization. Unexpectedly, both the catalytic alpha- and the non-catalytic beta-subunits comprise the characteristic fold of the class II active-site domains. The alpha beta heterodimer contains most of the building blocks so far identified in the class II synthetases. The presence of an RNA-binding domain, similar to that of the U1A spliceosomal protein, in the beta-subunit is indicative of structural relationships among different families of RNA-binding proteins. The structure suggests a plausible catalytic mechanism which explains why the primary site of tRNA aminoacylation is different from that of the other class II enzymes.
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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==About this Structure==
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[[Category: Delarue M]]
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1PYS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PYS OCA].
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[[Category: Goldgur Y]]
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[[Category: Mosyak L]]
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==Reference==
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[[Category: Reshetnikova L]]
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Structure of phenylalanyl-tRNA synthetase from Thermus thermophilus., Mosyak L, Reshetnikova L, Goldgur Y, Delarue M, Safro MG, Nat Struct Biol. 1995 Jul;2(7):537-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7664121 7664121]
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[[Category: Safro M]]
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[[Category: Phenylalanine--tRNA ligase]]
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[[Category: Protein complex]]
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[[Category: Thermus thermophilus]]
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[[Category: Delarue, M.]]
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[[Category: Goldgur, Y.]]
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[[Category: Mosyak, L.]]
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[[Category: Reshetnikova, L.]]
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[[Category: Safro, M.]]
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[[Category: MG]]
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[[Category: class ii aminoacyl-trna synthetase]]
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[[Category: helix-turn-helix motif]]
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[[Category: phenylalanyl-trna synthetase]]
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[[Category: rbd domain]]
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[[Category: sh3 domain]]
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[[Category: thermus thermophilus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:30:44 2008''
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Current revision

PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS

PDB ID 1pys

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