|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==Crystal structure of Rv3671c protease, inactive form== | | ==Crystal structure of Rv3671c protease, inactive form== |
| - | <StructureSection load='3k6z' size='340' side='right' caption='[[3k6z]], [[Resolution|resolution]] 1.75Å' scene=''> | + | <StructureSection load='3k6z' size='340' side='right'caption='[[3k6z]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3k6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K6Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3k6z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K6Z FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k6y|3k6y]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT3772, Rv3671c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k6z OCA], [https://pdbe.org/3k6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k6z RCSB], [https://www.ebi.ac.uk/pdbsum/3k6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k6z ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k6z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k6z RCSB], [http://www.ebi.ac.uk/pdbsum/3k6z PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Y3671_MYCTU Y3671_MYCTU] Required for M.tuberculosis resistance to oxidative stress in addition to its role in resistance to acid, which is essential for virulence. It protects M.tuberculosis against phagolysosomal concentrations of acid and maintains its intrabacterial pH when phagocytosed by IFN-gamma-activated macrophages.<ref>PMID:18641659</ref> <ref>PMID:20947023</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 15: |
Line 17: |
| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3k6z" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Mycobacterium tuberculosis]] | | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Biswas, T]] | + | [[Category: Biswas T]] |
| - | [[Category: Ehrt, S]] | + | [[Category: Ehrt S]] |
| - | [[Category: Small, J]] | + | [[Category: Small J]] |
| - | [[Category: Tsodikov, O V]] | + | [[Category: Tsodikov OV]] |
| - | [[Category: Vandal, O]] | + | [[Category: Vandal O]] |
| - | [[Category: Bent helix]]
| + | |
| - | [[Category: Disulfide]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Oxidative stress]]
| + | |
| - | [[Category: Protease]]
| + | |
| - | [[Category: Serine protease]]
| + | |
| Structural highlights
Function
Y3671_MYCTU Required for M.tuberculosis resistance to oxidative stress in addition to its role in resistance to acid, which is essential for virulence. It protects M.tuberculosis against phagolysosomal concentrations of acid and maintains its intrabacterial pH when phagocytosed by IFN-gamma-activated macrophages.[1] [2]
Publication Abstract from PubMed
Rv3671c, a putative serine protease, is crucial for persistence of Mycobacterium tuberculosis in the hostile environment of the phagosome. We show that Rv3671c is required for M. tuberculosis resistance to oxidative stress in addition to its role in protection from acidification. Structural and biochemical analyses demonstrate that the periplasmic domain of Rv3671c is a functional serine protease of the chymotrypsin family and, remarkably, that its activity increases on oxidation. High-resolution crystal structures of this protease in an active strained state and in an inactive relaxed state reveal that a solvent-exposed disulfide bond controls the protease activity by constraining two distant regions of Rv3671c and stabilizing it in the catalytically active conformation. In vitro biochemical studies confirm that activation of the protease in an oxidative environment is dependent on this reversible disulfide bond. These results suggest that the disulfide bond modulates activity of Rv3671c depending on the oxidative environment in vivo.
Structural insight into serine protease Rv3671c that Protects M. tuberculosis from oxidative and acidic stress.,Biswas T, Small J, Vandal O, Odaira T, Deng H, Ehrt S, Tsodikov OV Structure. 2010 Oct 13;18(10):1353-63. PMID:20947023[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vandal OH, Pierini LM, Schnappinger D, Nathan CF, Ehrt S. A membrane protein preserves intrabacterial pH in intraphagosomal Mycobacterium tuberculosis. Nat Med. 2008 Aug;14(8):849-54. doi: 10.1038/nm.1795. Epub 2008 Jul 20. PMID:18641659 doi:http://dx.doi.org/10.1038/nm.1795
- ↑ Biswas T, Small J, Vandal O, Odaira T, Deng H, Ehrt S, Tsodikov OV. Structural insight into serine protease Rv3671c that Protects M. tuberculosis from oxidative and acidic stress. Structure. 2010 Oct 13;18(10):1353-63. PMID:20947023 doi:10.1016/j.str.2010.06.017
- ↑ Biswas T, Small J, Vandal O, Odaira T, Deng H, Ehrt S, Tsodikov OV. Structural insight into serine protease Rv3671c that Protects M. tuberculosis from oxidative and acidic stress. Structure. 2010 Oct 13;18(10):1353-63. PMID:20947023 doi:10.1016/j.str.2010.06.017
|
