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3mq0

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==Crystal Structure of Agobacterium tumefaciens repressor BlcR==
==Crystal Structure of Agobacterium tumefaciens repressor BlcR==
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<StructureSection load='3mq0' size='340' side='right' caption='[[3mq0]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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<StructureSection load='3mq0' size='340' side='right'caption='[[3mq0]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3mq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MQ0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3mq0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MQ0 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AGR_pAT_196, attJ, Atu5136, blcR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=358 Agrobacterium tumefaciens])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.793&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mq0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mq0 RCSB], [http://www.ebi.ac.uk/pdbsum/3mq0 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mq0 OCA], [https://pdbe.org/3mq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mq0 RCSB], [https://www.ebi.ac.uk/pdbsum/3mq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mq0 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8VPD8_RHIRD Q8VPD8_RHIRD]
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The Agrobacterium tumefaciens BlcR is a member of the emerging isocitrate lyase transcription regulators that negatively regulates metabolism of gamma-butyrolactone, and its repressing function is relieved by succinate semialdehyde (SSA). Our crystal structure showed that BlcR folded into the DNA- and SSA-binding domains and dimerized via the DNA-binding domains. Mutational analysis identified residues, including Phe(147), that are important for SSA association; BlcR(F147A) existed as tetramer. Two BlcR dimers bound to target DNA and in a cooperative manner, and the distance between the two BlcR-binding sequences in DNA was critical for BlcR-DNA association. Tetrameric BlcR(F147A) retained DNA binding activity, and importantly, this activity was not affected by the distance separating the BlcR-binding sequences in DNA. SSA did not dissociate tetrameric BlcR(F147A) or BlcR(F147A)-DNA. As well as in the SSA-binding site, Phe(147) is located in a structurally flexible loop that may be involved in BlcR oligomerization. We propose that SSA regulates BlcR DNA-binding function via oligomerization.
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The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization.,Pan Y, Fiscus V, Meng W, Zheng Z, Zhang LH, Fuqua C, Chen L J Biol Chem. 2011 Jun 10;286(23):20431-40. Epub 2011 Apr 4. PMID:21467043<ref>PMID:21467043</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Chen, L]]
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[[Category: Large Structures]]
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[[Category: Gaf fold]]
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[[Category: Chen L]]
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[[Category: Helix-turn-helix]]
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[[Category: Transcription repressor]]
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Current revision

Crystal Structure of Agobacterium tumefaciens repressor BlcR

PDB ID 3mq0

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