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3nz3

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==Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae==
==Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae==
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<StructureSection load='3nz3' size='340' side='right' caption='[[3nz3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='3nz3' size='340' side='right'caption='[[3nz3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3nz3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NZ3 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3nz3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NZ3 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spr1345 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nz3 RCSB], [http://www.ebi.ac.uk/pdbsum/3nz3 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nz3 OCA], [https://pdbe.org/3nz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nz3 RCSB], [https://www.ebi.ac.uk/pdbsum/3nz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nz3 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8CYK3_STRR6 Q8CYK3_STRR6]
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The surface protein Spr1345 from Streptococcus pneumoniae R6 is a 22-kDa mucin-binding protein (MucBP) involved in adherence and colonization of the human lung and respiratory tract. It is composed of a mucin-binding domain (MucBD) and a proline-rich domain (PRD) followed by an LPxTG motif, which is recognized and cleaved by sortase, resulting in a mature form of 171 residues (MF171) that is anchored to the cell wall. We found that the MucBD alone possesses comparable in vitro mucin-binding affinity to the mature form, and can be specifically enriched at the surface of human lung carcinoma A549 cells. Using single-wavelength anomalous dispersion (SAD) phasing method with the iodine signals, we solved the crystal structure of the MucBD at 2.0A resolution, the first structure of MucBDs from pathogenic bacteria. The overall structure adopts an immunoglobulin-like fold with an elongated rod-like shape, composed of six anti-parallel beta-strands and a long loop. Structural comparison suggested that the conserved C-terminal moiety may participate in the recognition of mucins. These findings provided structural insights into host-pathogen interaction mediated by mucins, which might be useful for designing novel vaccines and antibiotic drugs against human diseases caused by pneumococci.
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Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae.,Du Y, He YX, Zhang ZY, Yang YH, Shi WW, Frolet C, Guilmi AM, Vernet T, Zhou CZ, Chen Y J Struct Biol. 2011 Apr;174(1):252-7. Epub 2010 Nov 3. PMID:21055474<ref>PMID:21055474</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Streptococcus pneumoniae]]
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[[Category: Large Structures]]
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[[Category: Chen, Y]]
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[[Category: Streptococcus pneumoniae R6]]
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[[Category: Du, Y]]
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[[Category: Chen Y]]
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[[Category: Frolet, C]]
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[[Category: Du Y]]
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[[Category: Guilmi, A M]]
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[[Category: Frolet C]]
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[[Category: He, Y X]]
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[[Category: Guilmi AM]]
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[[Category: Shi, W W]]
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[[Category: He Y-X]]
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[[Category: Vernet, T]]
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[[Category: Shi W-W]]
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[[Category: Yang, Y H]]
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[[Category: Vernet T]]
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[[Category: Zhang, Z Y]]
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[[Category: Yang Y-H]]
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[[Category: Zhou, C Z]]
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[[Category: Zhang Z-Y]]
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[[Category: All beta sheet]]
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[[Category: Zhou C-Z]]
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[[Category: Cell adhesion]]
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[[Category: Cell surface]]
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[[Category: Ig-like fold]]
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[[Category: Mucin]]
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[[Category: Mucin-binding]]
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Current revision

Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae

PDB ID 3nz3

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